Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7114 | 3' | -59 | NC_001895.1 | + | 13690 | 0.67 | 0.338111 |
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Target: 5'- --uUCGggUCAUUGcCCAGcGCCGCCGCCa -3' miRNA: 3'- gguGGU--GGUGAU-GGUCuCGGCGGCGG- -5' |
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| 7114 | 3' | -59 | NC_001895.1 | + | 18237 | 0.76 | 0.087102 |
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Target: 5'- aCCGCCACCGCguaUGCCaccgcccgugcgcuGGGGCUGCgCGCUa -3' miRNA: 3'- -GGUGGUGGUG---AUGG--------------UCUCGGCG-GCGG- -5' |
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| 7114 | 3' | -59 | NC_001895.1 | + | 20718 | 0.72 | 0.154344 |
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Target: 5'- gCCAgCGCCAUguucagcgugACCGGcGuuGCCGCCu -3' miRNA: 3'- -GGUgGUGGUGa---------UGGUCuCggCGGCGG- -5' |
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| 7114 | 3' | -59 | NC_001895.1 | + | 21155 | 0.68 | 0.284256 |
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Target: 5'- gCCAgCucaGCCGCUGCCGugcuGAuGaCUGCCGCCu -3' miRNA: 3'- -GGUgG---UGGUGAUGGU----CU-C-GGCGGCGG- -5' |
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| 7114 | 3' | -59 | NC_001895.1 | + | 21665 | 0.66 | 0.372065 |
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Target: 5'- aCCACgGCCACaACCGGccAGCUaauaGCC-CCg -3' miRNA: 3'- -GGUGgUGGUGaUGGUC--UCGG----CGGcGG- -5' |
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| 7114 | 3' | -59 | NC_001895.1 | + | 21994 | 0.69 | 0.243685 |
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Target: 5'- -gACaCACUug-ACCAGA-CCGCCGCCa -3' miRNA: 3'- ggUG-GUGGugaUGGUCUcGGCGGCGG- -5' |
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| 7114 | 3' | -59 | NC_001895.1 | + | 25013 | 0.66 | 0.389892 |
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Target: 5'- cUCACCAaagaauaacuCCGCUucaAUCAGGGCUguaaacauGCCGCUa -3' miRNA: 3'- -GGUGGU----------GGUGA---UGGUCUCGG--------CGGCGG- -5' |
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| 7114 | 3' | -59 | NC_001895.1 | + | 28919 | 0.71 | 0.181871 |
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Target: 5'- ---aCACCAUUGCCGguauuGAGCguuCGCCGCCg -3' miRNA: 3'- ggugGUGGUGAUGGU-----CUCG---GCGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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