Results 1 - 20 of 33 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7122 | 3' | -57.8 | NC_001895.1 | + | 8281 | 0.66 | 0.4714 |
Target: 5'- cGCACaGCAUCaggcaggucaGCACCGuaccaGCGGCg -3' miRNA: 3'- cCGUGcCGUAGg---------UGUGGCcaa--CGCCG- -5' |
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7122 | 3' | -57.8 | NC_001895.1 | + | 11566 | 0.66 | 0.461239 |
Target: 5'- cGGCACaGGCcagCAuCACCGcccgUGCGGCc -3' miRNA: 3'- -CCGUG-CCGuagGU-GUGGCca--ACGCCG- -5' |
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7122 | 3' | -57.8 | NC_001895.1 | + | 12638 | 0.66 | 0.461239 |
Target: 5'- gGGaCAuuccCGGCuAUCUguuCGCCGGUggUGUGGCu -3' miRNA: 3'- -CC-GU----GCCG-UAGGu--GUGGCCA--ACGCCG- -5' |
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7122 | 3' | -57.8 | NC_001895.1 | + | 20057 | 0.66 | 0.451196 |
Target: 5'- aGuCACGGCGagCUguGCACUGGUcuUGCGGa -3' miRNA: 3'- cC-GUGCCGUa-GG--UGUGGCCA--ACGCCg -5' |
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7122 | 3' | -57.8 | NC_001895.1 | + | 5172 | 0.66 | 0.451196 |
Target: 5'- cGGCucuGCGGUcgCCuuaGCCGG-UGUGGa -3' miRNA: 3'- -CCG---UGCCGuaGGug-UGGCCaACGCCg -5' |
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7122 | 3' | -57.8 | NC_001895.1 | + | 5911 | 0.66 | 0.441273 |
Target: 5'- cGCGCcagGGCGg-CACGCCGGUcagUGaCGGUg -3' miRNA: 3'- cCGUG---CCGUagGUGUGGCCA---AC-GCCG- -5' |
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7122 | 3' | -57.8 | NC_001895.1 | + | 21256 | 0.66 | 0.431476 |
Target: 5'- cGCAgCGGUcaguuuGUCCAgCuuuCCGGUugagGCGGCa -3' miRNA: 3'- cCGU-GCCG------UAGGU-Gu--GGCCAa---CGCCG- -5' |
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7122 | 3' | -57.8 | NC_001895.1 | + | 18299 | 0.66 | 0.431476 |
Target: 5'- cGCACgGGCGguggCAUACgCGGUgGCGGUg -3' miRNA: 3'- cCGUG-CCGUag--GUGUG-GCCAaCGCCG- -5' |
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7122 | 3' | -57.8 | NC_001895.1 | + | 7871 | 0.66 | 0.431476 |
Target: 5'- aGCGCGGUaaaaAUCCAC-CCGGcaGCGaccaGCg -3' miRNA: 3'- cCGUGCCG----UAGGUGuGGCCaaCGC----CG- -5' |
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7122 | 3' | -57.8 | NC_001895.1 | + | 21558 | 0.67 | 0.414166 |
Target: 5'- gGGCAgucuucacgacgguUGGCAgugcuguuaugaccgCCAUcggggcuauuagcugGCCGGUUGUGGCc -3' miRNA: 3'- -CCGU--------------GCCGUa--------------GGUG---------------UGGCCAACGCCG- -5' |
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7122 | 3' | -57.8 | NC_001895.1 | + | 10824 | 0.67 | 0.412269 |
Target: 5'- uGCGCGGCAacauugaaCACACCG---GCGGUg -3' miRNA: 3'- cCGUGCCGUag------GUGUGGCcaaCGCCG- -5' |
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7122 | 3' | -57.8 | NC_001895.1 | + | 18884 | 0.67 | 0.412269 |
Target: 5'- uGCGCGGCAUUgcCAUCGGUca-GGCu -3' miRNA: 3'- cCGUGCCGUAGguGUGGCCAacgCCG- -5' |
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7122 | 3' | -57.8 | NC_001895.1 | + | 29024 | 0.67 | 0.411323 |
Target: 5'- aGGCGCGGUaagcgugauaaacAUCCccacauCACCcucUGCGGCg -3' miRNA: 3'- -CCGUGCCG-------------UAGGu-----GUGGccaACGCCG- -5' |
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7122 | 3' | -57.8 | NC_001895.1 | + | 20887 | 0.67 | 0.406611 |
Target: 5'- aGGUGCGGCAUaggucagcgcaucgcUCAUGCCGucaaaaucgGCGGCg -3' miRNA: 3'- -CCGUGCCGUA---------------GGUGUGGCcaa------CGCCG- -5' |
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7122 | 3' | -57.8 | NC_001895.1 | + | 6419 | 0.67 | 0.402866 |
Target: 5'- cGCACuGGCAUCCACACCGcGauaacGCu -3' miRNA: 3'- cCGUG-CCGUAGGUGUGGC-CaacgcCG- -5' |
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7122 | 3' | -57.8 | NC_001895.1 | + | 6904 | 0.67 | 0.3936 |
Target: 5'- cGGCAuCGGC-UCCAUGCUGGguaucGcCGGUu -3' miRNA: 3'- -CCGU-GCCGuAGGUGUGGCCaa---C-GCCG- -5' |
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7122 | 3' | -57.8 | NC_001895.1 | + | 6117 | 0.67 | 0.3936 |
Target: 5'- -uCAcCGGCucgUCGCcuGCCGGUUGCGGg -3' miRNA: 3'- ccGU-GCCGua-GGUG--UGGCCAACGCCg -5' |
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7122 | 3' | -57.8 | NC_001895.1 | + | 7390 | 0.67 | 0.384474 |
Target: 5'- -uCugGGCGUCCcUGCCGGgUGcCGGUu -3' miRNA: 3'- ccGugCCGUAGGuGUGGCCaAC-GCCG- -5' |
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7122 | 3' | -57.8 | NC_001895.1 | + | 1009 | 0.67 | 0.381763 |
Target: 5'- uGCACGGCAgcCCGCaggcuuuuugccagACCGGUaaagcugaccgGUGGCu -3' miRNA: 3'- cCGUGCCGUa-GGUG--------------UGGCCAa----------CGCCG- -5' |
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7122 | 3' | -57.8 | NC_001895.1 | + | 1745 | 0.67 | 0.366646 |
Target: 5'- uGGUACuGGCA-CC--GCCGGUgGUGGCc -3' miRNA: 3'- -CCGUG-CCGUaGGugUGGCCAaCGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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