Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7163 | 3' | -57.4 | NC_001900.1 | + | 45660 | 1.1 | 0.000419 |
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Target: 5'- uGGCCCCGGUGAUGUACACCUGGUGCAg -3' miRNA: 3'- -CCGGGGCCACUACAUGUGGACCACGU- -5' |
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| 7163 | 3' | -57.4 | NC_001900.1 | + | 3544 | 0.69 | 0.386216 |
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Target: 5'- cGGCgCUCGGggccgGUAacgGCCUGGUGCAu -3' miRNA: 3'- -CCG-GGGCCacua-CAUg--UGGACCACGU- -5' |
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| 7163 | 3' | -57.4 | NC_001900.1 | + | 27037 | 0.69 | 0.395209 |
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Target: 5'- uGGCCCugagccgacaCGGUug-GUACGCCUGG-GCGc -3' miRNA: 3'- -CCGGG----------GCCAcuaCAUGUGGACCaCGU- -5' |
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| 7163 | 3' | -57.4 | NC_001900.1 | + | 48983 | 0.69 | 0.404335 |
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Target: 5'- uGGCuCCUGGUGGcucacaGCGCCUGG-GCGa -3' miRNA: 3'- -CCG-GGGCCACUaca---UGUGGACCaCGU- -5' |
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| 7163 | 3' | -57.4 | NC_001900.1 | + | 27493 | 0.68 | 0.413594 |
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Target: 5'- cGGCCgUCGGcGAUGUagcggcGCACCgucuUGGUGCu -3' miRNA: 3'- -CCGG-GGCCaCUACA------UGUGG----ACCACGu -5' |
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| 7163 | 3' | -57.4 | NC_001900.1 | + | 21202 | 0.68 | 0.461768 |
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Target: 5'- cGGCCCUGGUGA---ACAUC-GGUGgCGa -3' miRNA: 3'- -CCGGGGCCACUacaUGUGGaCCAC-GU- -5' |
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| 7163 | 3' | -57.4 | NC_001900.1 | + | 28430 | 0.68 | 0.461768 |
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Target: 5'- uGGCCUCcGUGGUGUgcgaccACugCUGGUacaGCAc -3' miRNA: 3'- -CCGGGGcCACUACA------UGugGACCA---CGU- -5' |
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| 7163 | 3' | -57.4 | NC_001900.1 | + | 1226 | 0.66 | 0.533778 |
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Target: 5'- aGGCaCCGGUGGUGUACucggUGGcgGCGg -3' miRNA: 3'- -CCGgGGCCACUACAUGugg-ACCa-CGU- -5' |
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| 7163 | 3' | -57.4 | NC_001900.1 | + | 11057 | 0.66 | 0.533778 |
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Target: 5'- cGCCaCCGGgcgcGAaacaUGCGCCUGGUGUc -3' miRNA: 3'- cCGG-GGCCa---CUac--AUGUGGACCACGu -5' |
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| 7163 | 3' | -57.4 | NC_001900.1 | + | 42912 | 0.66 | 0.576694 |
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Target: 5'- aGGCUCCauuccuGGcGAUGUACGCCUGuagGUAc -3' miRNA: 3'- -CCGGGG------CCaCUACAUGUGGACca-CGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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