Results 1 - 20 of 36 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7164 | 3' | -62.2 | NC_001900.1 | + | 24579 | 0.66 | 0.356298 |
Target: 5'- cACCGGGUCGaacGCUGGCCacaccuggucguugCCGAG-GCu -3' miRNA: 3'- -UGGUCCGGCaa-UGGCCGG--------------GGCUCgCG- -5' |
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7164 | 3' | -62.2 | NC_001900.1 | + | 42773 | 0.66 | 0.35875 |
Target: 5'- cACCAGGUCG-UGCCaGGCguccagCCCGA-CGUg -3' miRNA: 3'- -UGGUCCGGCaAUGG-CCG------GGGCUcGCG- -5' |
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7164 | 3' | -62.2 | NC_001900.1 | + | 9386 | 0.66 | 0.35875 |
Target: 5'- -aCAGGCUGUcgaggguuuCCGGCUCgGGGaCGCc -3' miRNA: 3'- ugGUCCGGCAau-------GGCCGGGgCUC-GCG- -5' |
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7164 | 3' | -62.2 | NC_001900.1 | + | 4547 | 0.66 | 0.35875 |
Target: 5'- gUCAGGuCCGUacaaCGGCCagcgagacaUCGAGCGCc -3' miRNA: 3'- uGGUCC-GGCAaug-GCCGG---------GGCUCGCG- -5' |
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7164 | 3' | -62.2 | NC_001900.1 | + | 36486 | 0.66 | 0.327029 |
Target: 5'- gGCuCAGGCCGUU-CaCGGCCaCCuGGuCGUa -3' miRNA: 3'- -UG-GUCCGGCAAuG-GCCGG-GGcUC-GCG- -5' |
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7164 | 3' | -62.2 | NC_001900.1 | + | 44298 | 0.67 | 0.311966 |
Target: 5'- cGCCccGCCGUaGCCGuaccuguuGCCagcgCGAGCGCg -3' miRNA: 3'- -UGGucCGGCAaUGGC--------CGGg---GCUCGCG- -5' |
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7164 | 3' | -62.2 | NC_001900.1 | + | 18664 | 0.67 | 0.319431 |
Target: 5'- gGCC-GGCCGUg--CGGCgCUGcaGGCGCa -3' miRNA: 3'- -UGGuCCGGCAaugGCCGgGGC--UCGCG- -5' |
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7164 | 3' | -62.2 | NC_001900.1 | + | 30526 | 0.67 | 0.319431 |
Target: 5'- uCCGGGCCGUgaagACCGuGUCCauccccGCGUa -3' miRNA: 3'- uGGUCCGGCAa---UGGC-CGGGgcu---CGCG- -5' |
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7164 | 3' | -62.2 | NC_001900.1 | + | 2432 | 0.67 | 0.317927 |
Target: 5'- uGCCGgguGGCUGUgugaggucgaCGGCCCCGGcuGCGUc -3' miRNA: 3'- -UGGU---CCGGCAaug-------GCCGGGGCU--CGCG- -5' |
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7164 | 3' | -62.2 | NC_001900.1 | + | 25740 | 0.67 | 0.304634 |
Target: 5'- uUCucGCCGacgcggGCCGcGCCCCG-GCGCa -3' miRNA: 3'- uGGucCGGCaa----UGGC-CGGGGCuCGCG- -5' |
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7164 | 3' | -62.2 | NC_001900.1 | + | 37513 | 0.67 | 0.319431 |
Target: 5'- cACCgGGGUCGggucGCCGG-CCgGAGUGCc -3' miRNA: 3'- -UGG-UCCGGCaa--UGGCCgGGgCUCGCG- -5' |
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7164 | 3' | -62.2 | NC_001900.1 | + | 25219 | 0.67 | 0.297435 |
Target: 5'- gGCCuGGCC-UUcCCaGCCCC-AGCGCc -3' miRNA: 3'- -UGGuCCGGcAAuGGcCGGGGcUCGCG- -5' |
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7164 | 3' | -62.2 | NC_001900.1 | + | 5977 | 0.68 | 0.276632 |
Target: 5'- gGCCAGGCCGcagcgGCUGaUCCUGGGCa- -3' miRNA: 3'- -UGGUCCGGCaa---UGGCcGGGGCUCGcg -5' |
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7164 | 3' | -62.2 | NC_001900.1 | + | 47223 | 0.68 | 0.269961 |
Target: 5'- gGCCgAGGCCGUcacCCGGCUuuCCGGGgaacaCGCu -3' miRNA: 3'- -UGG-UCCGGCAau-GGCCGG--GGCUC-----GCG- -5' |
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7164 | 3' | -62.2 | NC_001900.1 | + | 47853 | 0.68 | 0.250724 |
Target: 5'- cACCGGuGCCugcGUUGCCGgugccuaaaccGCCCCGGcCGCu -3' miRNA: 3'- -UGGUC-CGG---CAAUGGC-----------CGGGGCUcGCG- -5' |
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7164 | 3' | -62.2 | NC_001900.1 | + | 16818 | 0.68 | 0.244568 |
Target: 5'- cGCCGauGGUCGUcACgGGCaaccaCCGGGCGUg -3' miRNA: 3'- -UGGU--CCGGCAaUGgCCGg----GGCUCGCG- -5' |
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7164 | 3' | -62.2 | NC_001900.1 | + | 27463 | 0.68 | 0.276632 |
Target: 5'- aGCCugGGGCCGagccGCaCGGCCuuGAGCcgGCc -3' miRNA: 3'- -UGG--UCCGGCaa--UG-GCCGGggCUCG--CG- -5' |
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7164 | 3' | -62.2 | NC_001900.1 | + | 5630 | 0.68 | 0.269961 |
Target: 5'- cAUCGGuGCCGccuggUACUGGaCCgUCGAGCGCc -3' miRNA: 3'- -UGGUC-CGGCa----AUGGCC-GG-GGCUCGCG- -5' |
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7164 | 3' | -62.2 | NC_001900.1 | + | 4175 | 0.68 | 0.269961 |
Target: 5'- cUCGGcGUCGUcUACCGGCaacCCCGguaGGCGCa -3' miRNA: 3'- uGGUC-CGGCA-AUGGCCG---GGGC---UCGCG- -5' |
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7164 | 3' | -62.2 | NC_001900.1 | + | 37263 | 0.68 | 0.26342 |
Target: 5'- cGCCgacaGGGCCGUcgUACaCGGCCCgcAGCGg -3' miRNA: 3'- -UGG----UCCGGCA--AUG-GCCGGGgcUCGCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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