Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7166 | 5' | -53.8 | NC_001900.1 | + | 9513 | 0.66 | 0.79201 |
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Target: 5'- aGAGCCGCGAAG-AGgacucCGGG-GUaccGGGu -3' miRNA: 3'- aCUUGGUGCUUCaUCa----GCCCgCA---CCC- -5' |
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| 7166 | 5' | -53.8 | NC_001900.1 | + | 18513 | 0.66 | 0.791025 |
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Target: 5'- cUGAGCUgucucagGCGAuggAGUCuGGCGUGGa -3' miRNA: 3'- -ACUUGG-------UGCUucaUCAGcCCGCACCc -5' |
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| 7166 | 5' | -53.8 | NC_001900.1 | + | 33827 | 0.67 | 0.719591 |
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Target: 5'- aGGACCACGAcGUAGUCGaucGGauccucgGUGGu -3' miRNA: 3'- aCUUGGUGCUuCAUCAGC---CCg------CACCc -5' |
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| 7166 | 5' | -53.8 | NC_001900.1 | + | 5108 | 0.67 | 0.719591 |
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Target: 5'- gGAGuCC-CGAGGagaAGUCaacGGCGUGGGg -3' miRNA: 3'- aCUU-GGuGCUUCa--UCAGc--CCGCACCC- -5' |
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| 7166 | 5' | -53.8 | NC_001900.1 | + | 20495 | 0.69 | 0.620666 |
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Target: 5'- -cAACCACGAcGgcgAGU-GGGCGUGGc -3' miRNA: 3'- acUUGGUGCUuCa--UCAgCCCGCACCc -5' |
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| 7166 | 5' | -53.8 | NC_001900.1 | + | 40053 | 0.7 | 0.576505 |
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Target: 5'- cGAAUCGCGAuggcgucguaGGUAGUUGGGUGUc-- -3' miRNA: 3'- aCUUGGUGCU----------UCAUCAGCCCGCAccc -5' |
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| 7166 | 5' | -53.8 | NC_001900.1 | + | 47373 | 0.71 | 0.522399 |
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Target: 5'- cGAACUccagcGCGGAGUAGaccggCGGGaCGUGGc -3' miRNA: 3'- aCUUGG-----UGCUUCAUCa----GCCC-GCACCc -5' |
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| 7166 | 5' | -53.8 | NC_001900.1 | + | 18962 | 0.71 | 0.467328 |
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Target: 5'- aGGACCGCGAGGaAuccaagaacgcgcuGUCGGuCGUGGGc -3' miRNA: 3'- aCUUGGUGCUUCaU--------------CAGCCcGCACCC- -5' |
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| 7166 | 5' | -53.8 | NC_001900.1 | + | 21950 | 0.73 | 0.393083 |
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Target: 5'- aUGAGCCGUGAGGc---UGGGCGUGGGc -3' miRNA: 3'- -ACUUGGUGCUUCaucaGCCCGCACCC- -5' |
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| 7166 | 5' | -53.8 | NC_001900.1 | + | 43492 | 1.12 | 0.000915 |
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Target: 5'- gUGAACCACGAAGUAGUCGGGCGUGGGg -3' miRNA: 3'- -ACUUGGUGCUUCAUCAGCCCGCACCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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