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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7169 | 3' | -52.8 | NC_001900.1 | + | 43635 | 0.67 | 0.763984 |
Target: 5'- -uGCC--GAGGuCGGUUCGGGU-GUCGu -3' miRNA: 3'- uuUGGaaCUCC-GCCAAGCCUAgCAGU- -5' |
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7169 | 3' | -52.8 | NC_001900.1 | + | 17405 | 0.68 | 0.653493 |
Target: 5'- -cACCgcGAGGCGGaccUUCGGugaagcguUCGUCAa -3' miRNA: 3'- uuUGGaaCUCCGCC---AAGCCu-------AGCAGU- -5' |
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7169 | 3' | -52.8 | NC_001900.1 | + | 41516 | 1.06 | 0.002184 |
Target: 5'- aAAACCUUGAGGCGGUUCGGAUCGUCAu -3' miRNA: 3'- -UUUGGAACUCCGCCAAGCCUAGCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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