Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7171 | 5' | -63.8 | NC_001900.1 | + | 17475 | 0.71 | 0.136598 |
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Target: 5'- cGGCUcauggaguCCGGUCUgcaGGCGauGGGUCAGCUCGg -3' miRNA: 3'- -CCGG--------GGCCAGG---UCGC--CUCGGUCGAGC- -5' |
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| 7171 | 5' | -63.8 | NC_001900.1 | + | 17365 | 0.67 | 0.233699 |
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Target: 5'- -aCCUgCGGUCgGagaacgccgaucuGCGGAGUCAGCUCGa -3' miRNA: 3'- ccGGG-GCCAGgU-------------CGCCUCGGUCGAGC- -5' |
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| 7171 | 5' | -63.8 | NC_001900.1 | + | 10657 | 0.7 | 0.155767 |
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Target: 5'- gGGCUcgCCGGguacgUCCAGCGGuucGCCAGuCUCu -3' miRNA: 3'- -CCGG--GGCC-----AGGUCGCCu--CGGUC-GAGc -5' |
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| 7171 | 5' | -63.8 | NC_001900.1 | + | 9784 | 0.66 | 0.298676 |
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Target: 5'- cGGUCCCGGuggucccgcuUCCGaacCGGAcCCGGCUCu -3' miRNA: 3'- -CCGGGGCC----------AGGUc--GCCUcGGUCGAGc -5' |
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| 7171 | 5' | -63.8 | NC_001900.1 | + | 6476 | 0.67 | 0.258524 |
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Target: 5'- aGGCUCUGGagUCCGcugucaacggcGCGGucccCCAGCUCGg -3' miRNA: 3'- -CCGGGGCC--AGGU-----------CGCCuc--GGUCGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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