Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7173 | 5' | -59.6 | NC_001900.1 | + | 21081 | 0.66 | 0.521886 |
Target: 5'- gUCGuGUUC-GAAGaGGGGCCacugACCGGGa -3' miRNA: 3'- aGGC-CAAGcCUUCgCCCUGG----UGGCCC- -5' |
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7173 | 5' | -59.6 | NC_001900.1 | + | 13975 | 0.66 | 0.511765 |
Target: 5'- gUCCGGUacgUCGGcacgacgugcuGAGCaGGACaACgGGGg -3' miRNA: 3'- -AGGCCA---AGCC-----------UUCGcCCUGgUGgCCC- -5' |
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7173 | 5' | -59.6 | NC_001900.1 | + | 30000 | 0.66 | 0.511765 |
Target: 5'- gUCCaG-UCGGAcGCGGGcaGCgucugCGCCGGGa -3' miRNA: 3'- -AGGcCaAGCCUuCGCCC--UG-----GUGGCCC- -5' |
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7173 | 5' | -59.6 | NC_001900.1 | + | 10637 | 0.66 | 0.511765 |
Target: 5'- cCCaGgcCGuGAucacuGCGGGGCuCGCCGGGu -3' miRNA: 3'- aGGcCaaGC-CUu----CGCCCUG-GUGGCCC- -5' |
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7173 | 5' | -59.6 | NC_001900.1 | + | 1158 | 0.66 | 0.491776 |
Target: 5'- gUgGGUggacUUGGAGGCGGcGguggaGCCGCCGGa -3' miRNA: 3'- aGgCCA----AGCCUUCGCC-C-----UGGUGGCCc -5' |
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7173 | 5' | -59.6 | NC_001900.1 | + | 38526 | 0.66 | 0.481916 |
Target: 5'- --gGGUUgGgGGAGCcuGGGACCGUCGGGu -3' miRNA: 3'- aggCCAAgC-CUUCG--CCCUGGUGGCCC- -5' |
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7173 | 5' | -59.6 | NC_001900.1 | + | 25111 | 0.67 | 0.466345 |
Target: 5'- gCCGGUUUcGAGGUGgaccgagccgccaccGaGACCGCUGGGg -3' miRNA: 3'- aGGCCAAGcCUUCGC---------------C-CUGGUGGCCC- -5' |
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7173 | 5' | -59.6 | NC_001900.1 | + | 15999 | 0.67 | 0.462493 |
Target: 5'- cUCCGGUggcggcgguagCGGcGGCGGuGGCCAgcagcaccagucUCGGGc -3' miRNA: 3'- -AGGCCAa----------GCCuUCGCC-CUGGU------------GGCCC- -5' |
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7173 | 5' | -59.6 | NC_001900.1 | + | 46004 | 0.67 | 0.434153 |
Target: 5'- gCCGG-UCGGGucGGCGGGugC-CCGc- -3' miRNA: 3'- aGGCCaAGCCU--UCGCCCugGuGGCcc -5' |
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7173 | 5' | -59.6 | NC_001900.1 | + | 5260 | 0.67 | 0.419456 |
Target: 5'- uUCCGGUUcgcucgcgcucacagCGaGGAGCGGccCgACCGGGa -3' miRNA: 3'- -AGGCCAA---------------GC-CUUCGCCcuGgUGGCCC- -5' |
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7173 | 5' | -59.6 | NC_001900.1 | + | 19310 | 0.68 | 0.397987 |
Target: 5'- gCCGGgucuaCGGcGGUGGGGCCAacaCGGu -3' miRNA: 3'- aGGCCaa---GCCuUCGCCCUGGUg--GCCc -5' |
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7173 | 5' | -59.6 | NC_001900.1 | + | 10763 | 0.68 | 0.372163 |
Target: 5'- gUCGGUaugCGGAAGCugccGACCuggGCCGGGa -3' miRNA: 3'- aGGCCAa--GCCUUCGcc--CUGG---UGGCCC- -5' |
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7173 | 5' | -59.6 | NC_001900.1 | + | 24041 | 0.69 | 0.339547 |
Target: 5'- cCUGGccaCGGGcacCGGGACCACCGGc -3' miRNA: 3'- aGGCCaa-GCCUuc-GCCCUGGUGGCCc -5' |
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7173 | 5' | -59.6 | NC_001900.1 | + | 37480 | 0.74 | 0.16042 |
Target: 5'- cUUCGGagUCGGuuccAGCGGcuugucaguGACCACCGGGg -3' miRNA: 3'- -AGGCCa-AGCCu---UCGCC---------CUGGUGGCCC- -5' |
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7173 | 5' | -59.6 | NC_001900.1 | + | 39275 | 1.11 | 0.000328 |
Target: 5'- gUCCGGUUCGGAAGCGGGACCACCGGGa -3' miRNA: 3'- -AGGCCAAGCCUUCGCCCUGGUGGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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