Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7176 | 3' | -57.9 | NC_001900.1 | + | 25759 | 0.67 | 0.480221 |
Target: 5'- --gCCgggGCCGACCGCCGuGGUgUUGa -3' miRNA: 3'- caaGGa--CGGCUGGUGGCuUCGgGACc -5' |
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7176 | 3' | -57.9 | NC_001900.1 | + | 35094 | 0.67 | 0.499518 |
Target: 5'- -gUCgUGCCGACgUACCGGacugguuGGCgaCCUGGa -3' miRNA: 3'- caAGgACGGCUG-GUGGCU-------UCG--GGACC- -5' |
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7176 | 3' | -57.9 | NC_001900.1 | + | 13921 | 0.66 | 0.530655 |
Target: 5'- aUUCCUcGCUGACUACCcgcacguGAAGCaggagCUGGa -3' miRNA: 3'- cAAGGA-CGGCUGGUGG-------CUUCGg----GACC- -5' |
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7176 | 3' | -57.9 | NC_001900.1 | + | 20715 | 0.66 | 0.542251 |
Target: 5'- cUUCgCUGUCGACCagACCGAcggcagcaAGCUgUGGc -3' miRNA: 3'- cAAG-GACGGCUGG--UGGCU--------UCGGgACC- -5' |
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7176 | 3' | -57.9 | NC_001900.1 | + | 17467 | 0.66 | 0.563541 |
Target: 5'- --aCCgGCCGaACCGCCaGAAGCUCa-- -3' miRNA: 3'- caaGGaCGGC-UGGUGG-CUUCGGGacc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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