Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7176 | 3' | -57.9 | NC_001900.1 | + | 29737 | 0.69 | 0.359466 |
Target: 5'- --gUCUGCuCGGCCACCGuguuGGCCCc-- -3' miRNA: 3'- caaGGACG-GCUGGUGGCu---UCGGGacc -5' |
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7176 | 3' | -57.9 | NC_001900.1 | + | 4129 | 0.7 | 0.334772 |
Target: 5'- gGUUCauCUGCCGACCGgCGAAGCggCCggcGGu -3' miRNA: 3'- -CAAG--GACGGCUGGUgGCUUCG--GGa--CC- -5' |
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7176 | 3' | -57.9 | NC_001900.1 | + | 15943 | 0.71 | 0.296426 |
Target: 5'- cUUCCgGCUGAUCGCCacGAAGCCCa-- -3' miRNA: 3'- cAAGGaCGGCUGGUGG--CUUCGGGacc -5' |
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7176 | 3' | -57.9 | NC_001900.1 | + | 22075 | 0.75 | 0.142361 |
Target: 5'- aGUUCCaGCCGGCCuuCCGAGGCCagcGGc -3' miRNA: 3'- -CAAGGaCGGCUGGu-GGCUUCGGga-CC- -5' |
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7176 | 3' | -57.9 | NC_001900.1 | + | 15082 | 0.66 | 0.521235 |
Target: 5'- --aCCUGuCCGACCcgucgaccuggACUGGAGCCaccggCUGGu -3' miRNA: 3'- caaGGAC-GGCUGG-----------UGGCUUCGG-----GACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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