miRNA: 3'- -AGUGGuCGUCCGagUCCGGCAaGUGc -5'
miRNA: 3'- aGUGGUCgUCCgagUCCGGCAAGUGC- -5'
miRNA: 3'- aGUGGUCGuCCGAGUCCGGCaagUGC- -5'
miRNA: 3'- agUGGUCG-------UCCGAGUCCGGCaaGUGC- -5'
miRNA: 3'- -AGUGGUCGUCCGAGUCCGGCAAGUGC- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7178 | 3' | -57.4 | NC_001900.1 | + | 47213 | 0.73 | 0.205512 |
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Target: 5'- uUCGCCcGCAGGCcgAGGCCGU-CACc -3' miRNA: 3'- -AGUGGuCGUCCGagUCCGGCAaGUGc -5' |
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| 7178 | 3' | -57.4 | NC_001900.1 | + | 13054 | 0.74 | 0.17971 |
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Target: 5'- cCACCGGgAGG---GGGCCGUUCACGc -3' miRNA: 3'- aGUGGUCgUCCgagUCCGGCAAGUGC- -5' |
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| 7178 | 3' | -57.4 | NC_001900.1 | + | 23600 | 0.75 | 0.148427 |
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Target: 5'- aCGCUGGC-GGCUCAGGCCGgu-GCGa -3' miRNA: 3'- aGUGGUCGuCCGAGUCCGGCaagUGC- -5' |
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| 7178 | 3' | -57.4 | NC_001900.1 | + | 25153 | 0.81 | 0.061404 |
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Target: 5'- -aGCCAGCcucgcagAGGCUCGGGCCGggUACGa -3' miRNA: 3'- agUGGUCG-------UCCGAGUCCGGCaaGUGC- -5' |
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| 7178 | 3' | -57.4 | NC_001900.1 | + | 36476 | 1.1 | 0.00043 |
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Target: 5'- uUCACCAGCAGGCUCAGGCCGUUCACGg -3' miRNA: 3'- -AGUGGUCGUCCGAGUCCGGCAAGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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