Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7178 | 3' | -57.4 | NC_001900.1 | + | 5208 | 0.67 | 0.470677 |
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Target: 5'- gCACCAGUcGGCcCAgcccucgcgcuGGCCGUUCcCGa -3' miRNA: 3'- aGUGGUCGuCCGaGU-----------CCGGCAAGuGC- -5' |
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| 7178 | 3' | -57.4 | NC_001900.1 | + | 3532 | 0.67 | 0.491037 |
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Target: 5'- aCGCCAucgacGCGGcGCUCGGGgCCGguaACGg -3' miRNA: 3'- aGUGGU-----CGUC-CGAGUCC-GGCaagUGC- -5' |
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| 7178 | 3' | -57.4 | NC_001900.1 | + | 4014 | 0.67 | 0.499295 |
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Target: 5'- -aGCCAGgcaAGGaccgccugaccaUCuGGCCGUUCACGa -3' miRNA: 3'- agUGGUCg--UCCg-----------AGuCCGGCAAGUGC- -5' |
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| 7178 | 3' | -57.4 | NC_001900.1 | + | 41078 | 0.66 | 0.532908 |
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Target: 5'- -gACguuGCGGGC-CAGGCCGgugaccUCGCGg -3' miRNA: 3'- agUGgu-CGUCCGaGUCCGGCa-----AGUGC- -5' |
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| 7178 | 3' | -57.4 | NC_001900.1 | + | 7861 | 0.66 | 0.554325 |
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Target: 5'- gCACCAGCAGGU----GCUGggCGCGc -3' miRNA: 3'- aGUGGUCGUCCGagucCGGCaaGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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