Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7178 | 3' | -57.4 | NC_001900.1 | + | 46134 | 0.7 | 0.310153 |
Target: 5'- aUCGCCgAGCuGGUcgaugacCAGGCCGUUgCGCGa -3' miRNA: 3'- -AGUGG-UCGuCCGa------GUCCGGCAA-GUGC- -5' |
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7178 | 3' | -57.4 | NC_001900.1 | + | 17245 | 0.73 | 0.211044 |
Target: 5'- gCACUGGCgaguucuucaccGGGCUCGGGCCGgUCAUc -3' miRNA: 3'- aGUGGUCG------------UCCGAGUCCGGCaAGUGc -5' |
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7178 | 3' | -57.4 | NC_001900.1 | + | 13054 | 0.74 | 0.17971 |
Target: 5'- cCACCGGgAGG---GGGCCGUUCACGc -3' miRNA: 3'- aGUGGUCgUCCgagUCCGGCAAGUGC- -5' |
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7178 | 3' | -57.4 | NC_001900.1 | + | 25153 | 0.81 | 0.061404 |
Target: 5'- -aGCCAGCcucgcagAGGCUCGGGCCGggUACGa -3' miRNA: 3'- agUGGUCG-------UCCGAGUCCGGCaaGUGC- -5' |
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7178 | 3' | -57.4 | NC_001900.1 | + | 41078 | 0.66 | 0.532908 |
Target: 5'- -gACguuGCGGGC-CAGGCCGgugaccUCGCGg -3' miRNA: 3'- agUGgu-CGUCCGaGUCCGGCa-----AGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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