Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7180 | 3' | -56 | NC_001900.1 | + | 37050 | 0.66 | 0.635609 |
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Target: 5'- uGAUG-CCGAGaACGCCCuucaUGGCGUUgAg -3' miRNA: 3'- -CUACaGGCUUcUGUGGG----ACCGCAGgU- -5' |
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| 7180 | 3' | -56 | NC_001900.1 | + | 31745 | 0.66 | 0.613465 |
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Target: 5'- aGAUGUCCGA--ACGCgUUGGC-UCCu -3' miRNA: 3'- -CUACAGGCUucUGUGgGACCGcAGGu -5' |
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| 7180 | 3' | -56 | NC_001900.1 | + | 24321 | 0.67 | 0.591388 |
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Target: 5'- -cUGUCCGAAGAUguaaccagGCCCU-GCGguaCCAc -3' miRNA: 3'- cuACAGGCUUCUG--------UGGGAcCGCa--GGU- -5' |
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| 7180 | 3' | -56 | NC_001900.1 | + | 9995 | 0.67 | 0.580397 |
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Target: 5'- -cUGUUCGAcGcguaccucgcccGCAUCCUGGCGUUCGa -3' miRNA: 3'- cuACAGGCUuC------------UGUGGGACCGCAGGU- -5' |
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| 7180 | 3' | -56 | NC_001900.1 | + | 5631 | 0.67 | 0.547717 |
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Target: 5'- aGUGaUCCG-AGACAagaUCCUGGCGaUCCu -3' miRNA: 3'- cUAC-AGGCuUCUGU---GGGACCGC-AGGu -5' |
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| 7180 | 3' | -56 | NC_001900.1 | + | 17501 | 0.68 | 0.49468 |
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Target: 5'- --gGUCCG-GGACAUCCgggaGGCGUaCCGg -3' miRNA: 3'- cuaCAGGCuUCUGUGGGa---CCGCA-GGU- -5' |
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| 7180 | 3' | -56 | NC_001900.1 | + | 25809 | 0.69 | 0.444105 |
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Target: 5'- cGAUG-CCGguGugGUCCUGuGCGUCCAg -3' miRNA: 3'- -CUACaGGCuuCugUGGGAC-CGCAGGU- -5' |
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| 7180 | 3' | -56 | NC_001900.1 | + | 10389 | 0.76 | 0.175311 |
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Target: 5'- aGAUGUaCCG-AGGCugCCUGGCGaUCCc -3' miRNA: 3'- -CUACA-GGCuUCUGugGGACCGC-AGGu -5' |
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| 7180 | 3' | -56 | NC_001900.1 | + | 35668 | 1.09 | 0.000757 |
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Target: 5'- aGAUGUCCGAAGACACCCUGGCGUCCAg -3' miRNA: 3'- -CUACAGGCUUCUGUGGGACCGCAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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