Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7184 | 3' | -52.7 | NC_001900.1 | + | 31524 | 1.07 | 0.002324 |
Target: 5'- gCGCGAUGAACAGAUCACCGAUCCCGUu -3' miRNA: 3'- -GCGCUACUUGUCUAGUGGCUAGGGCA- -5' |
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7184 | 3' | -52.7 | NC_001900.1 | + | 19850 | 0.73 | 0.421003 |
Target: 5'- aCGCGAgGAGCAGAUCAgcuCCGAgucgggcucCCCGg -3' miRNA: 3'- -GCGCUaCUUGUCUAGU---GGCUa--------GGGCa -5' |
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7184 | 3' | -52.7 | NC_001900.1 | + | 43382 | 0.72 | 0.48117 |
Target: 5'- cCGCGAUGAAgAGGUCAUC---CCCGUc -3' miRNA: 3'- -GCGCUACUUgUCUAGUGGcuaGGGCA- -5' |
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7184 | 3' | -52.7 | NC_001900.1 | + | 41344 | 0.72 | 0.502145 |
Target: 5'- gGCGGUGAACGGGUCuuugcCCcAUCCCu- -3' miRNA: 3'- gCGCUACUUGUCUAGu----GGcUAGGGca -5' |
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7184 | 3' | -52.7 | NC_001900.1 | + | 29031 | 0.7 | 0.578282 |
Target: 5'- gGCGGUG--UAGA-CGCCGAUCUCGUc -3' miRNA: 3'- gCGCUACuuGUCUaGUGGCUAGGGCA- -5' |
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7184 | 3' | -52.7 | NC_001900.1 | + | 12479 | 0.69 | 0.65661 |
Target: 5'- gGCGAUGcACGG--CACCGAcagCCCGUu -3' miRNA: 3'- gCGCUACuUGUCuaGUGGCUa--GGGCA- -5' |
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7184 | 3' | -52.7 | NC_001900.1 | + | 27501 | 0.69 | 0.66779 |
Target: 5'- gGCGAUGuAGCGGcgCACCG-UCuuGg -3' miRNA: 3'- gCGCUAC-UUGUCuaGUGGCuAGggCa -5' |
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7184 | 3' | -52.7 | NC_001900.1 | + | 19893 | 0.68 | 0.701075 |
Target: 5'- gCGCgGAUGAACGG-UguCCGGUUCCGc -3' miRNA: 3'- -GCG-CUACUUGUCuAguGGCUAGGGCa -5' |
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7184 | 3' | -52.7 | NC_001900.1 | + | 36221 | 0.68 | 0.712046 |
Target: 5'- aCGuCGAaGGACAGAcCGCCGAUCUgGc -3' miRNA: 3'- -GC-GCUaCUUGUCUaGUGGCUAGGgCa -5' |
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7184 | 3' | -52.7 | NC_001900.1 | + | 27139 | 0.68 | 0.722936 |
Target: 5'- uCGCGAUGGu--GAUCGCggCGAUUCCGc -3' miRNA: 3'- -GCGCUACUuguCUAGUG--GCUAGGGCa -5' |
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7184 | 3' | -52.7 | NC_001900.1 | + | 42991 | 0.68 | 0.733732 |
Target: 5'- uGCGAUGAACucGGGggAgCGAUCCCu- -3' miRNA: 3'- gCGCUACUUG--UCUagUgGCUAGGGca -5' |
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7184 | 3' | -52.7 | NC_001900.1 | + | 23671 | 0.68 | 0.733732 |
Target: 5'- gGCGAUGAAguacCAGAUgGCCucagcagCCCGUg -3' miRNA: 3'- gCGCUACUU----GUCUAgUGGcua----GGGCA- -5' |
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7184 | 3' | -52.7 | NC_001900.1 | + | 640 | 0.68 | 0.733732 |
Target: 5'- aCGCGAUaGGCGGAUCggaaacgaucgcGCCGgAUCCgGUg -3' miRNA: 3'- -GCGCUAcUUGUCUAG------------UGGC-UAGGgCA- -5' |
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7184 | 3' | -52.7 | NC_001900.1 | + | 39940 | 0.68 | 0.743358 |
Target: 5'- gCGCGAacgaaccUGAGCccGGA-CACCGaAUCCCGc -3' miRNA: 3'- -GCGCU-------ACUUG--UCUaGUGGC-UAGGGCa -5' |
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7184 | 3' | -52.7 | NC_001900.1 | + | 39081 | 0.67 | 0.754994 |
Target: 5'- aCGCGucGAACAGcGUCuguCCGGUCUCGg -3' miRNA: 3'- -GCGCuaCUUGUC-UAGu--GGCUAGGGCa -5' |
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7184 | 3' | -52.7 | NC_001900.1 | + | 24255 | 0.66 | 0.80566 |
Target: 5'- aGCGAUGAGCGugcCACCG--CCCGa -3' miRNA: 3'- gCGCUACUUGUcuaGUGGCuaGGGCa -5' |
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7184 | 3' | -52.7 | NC_001900.1 | + | 5617 | 0.66 | 0.80566 |
Target: 5'- uGCGcUGGGCAGG-CAUCGGUgCCGc -3' miRNA: 3'- gCGCuACUUGUCUaGUGGCUAgGGCa -5' |
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7184 | 3' | -52.7 | NC_001900.1 | + | 42150 | 0.66 | 0.80566 |
Target: 5'- aGCGAcuccaggccgcUGAACGGGUCAuccCCGuccCCCGa -3' miRNA: 3'- gCGCU-----------ACUUGUCUAGU---GGCua-GGGCa -5' |
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7184 | 3' | -52.7 | NC_001900.1 | + | 26003 | 0.66 | 0.815273 |
Target: 5'- uCGCGGUGucccGCAGc-CACuCGAUCUCGUc -3' miRNA: 3'- -GCGCUACu---UGUCuaGUG-GCUAGGGCA- -5' |
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7184 | 3' | -52.7 | NC_001900.1 | + | 9190 | 0.66 | 0.824685 |
Target: 5'- aCGCGAUGuaccgccACGGcgUACCGAcCgCCGUg -3' miRNA: 3'- -GCGCUACu------UGUCuaGUGGCUaG-GGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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