miRNA: 3'- gCGCUACUUGUCUAGu----GGcUAGGGca -5'
miRNA: 3'- -GCGCUACUUgUCUAGUGGcuaGGGCA- -5'
miRNA: 3'- -GCGCUaCUUGUCUAGU---GGCUa--------GGGCa -5'
miRNA: 3'- -GCGCUACUUGUCUAGUGGCUAGGGCA- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7184 | 3' | -52.7 | NC_001900.1 | + | 41344 | 0.72 | 0.502145 |
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Target: 5'- gGCGGUGAACGGGUCuuugcCCcAUCCCu- -3' miRNA: 3'- gCGCUACUUGUCUAGu----GGcUAGGGca -5' |
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| 7184 | 3' | -52.7 | NC_001900.1 | + | 43382 | 0.72 | 0.48117 |
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Target: 5'- cCGCGAUGAAgAGGUCAUC---CCCGUc -3' miRNA: 3'- -GCGCUACUUgUCUAGUGGcuaGGGCA- -5' |
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| 7184 | 3' | -52.7 | NC_001900.1 | + | 19850 | 0.73 | 0.421003 |
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Target: 5'- aCGCGAgGAGCAGAUCAgcuCCGAgucgggcucCCCGg -3' miRNA: 3'- -GCGCUaCUUGUCUAGU---GGCUa--------GGGCa -5' |
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| 7184 | 3' | -52.7 | NC_001900.1 | + | 31524 | 1.07 | 0.002324 |
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Target: 5'- gCGCGAUGAACAGAUCACCGAUCCCGUu -3' miRNA: 3'- -GCGCUACUUGUCUAGUGGCUAGGGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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