Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7184 | 3' | -52.7 | NC_001900.1 | + | 10968 | 0.66 | 0.851621 |
Target: 5'- gCGCGAagUGGgaugGCAGGUCGCCGAcagaUCaUCGg -3' miRNA: 3'- -GCGCU--ACU----UGUCUAGUGGCU----AG-GGCa -5' |
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7184 | 3' | -52.7 | NC_001900.1 | + | 9190 | 0.66 | 0.824685 |
Target: 5'- aCGCGAUGuaccgccACGGcgUACCGAcCgCCGUg -3' miRNA: 3'- -GCGCUACu------UGUCuaGUGGCUaG-GGCA- -5' |
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7184 | 3' | -52.7 | NC_001900.1 | + | 5617 | 0.66 | 0.80566 |
Target: 5'- uGCGcUGGGCAGG-CAUCGGUgCCGc -3' miRNA: 3'- gCGCuACUUGUCUaGUGGCUAgGGCa -5' |
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7184 | 3' | -52.7 | NC_001900.1 | + | 640 | 0.68 | 0.733732 |
Target: 5'- aCGCGAUaGGCGGAUCggaaacgaucgcGCCGgAUCCgGUg -3' miRNA: 3'- -GCGCUAcUUGUCUAG------------UGGC-UAGGgCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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