Results 1 - 20 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7187 | 3' | -56.3 | NC_001900.1 | + | 15819 | 0.76 | 0.185194 |
Target: 5'- -gCGAAGCCGUgGUCGAGGCCgUCa- -3' miRNA: 3'- gaGCUUCGGCAaCGGCUUCGGgAGcc -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 20006 | 0.66 | 0.638888 |
Target: 5'- -cCGAGGCCGUggucgcgcUGCUGggG-UCUCGa -3' miRNA: 3'- gaGCUUCGGCA--------ACGGCuuCgGGAGCc -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 34478 | 0.66 | 0.617037 |
Target: 5'- -cCGGcucGCCGUUGCguaCGGcuCCCUCGGa -3' miRNA: 3'- gaGCUu--CGGCAACG---GCUucGGGAGCC- -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 17715 | 0.66 | 0.617037 |
Target: 5'- gCUCuGggGCCugUGCUGAccguggucgcugAGaCCCUCGGc -3' miRNA: 3'- -GAG-CuuCGGcaACGGCU------------UC-GGGAGCC- -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 35562 | 0.68 | 0.530834 |
Target: 5'- cCUUGAaauAGgCGUcaGCCGAAucGCCCUUGGa -3' miRNA: 3'- -GAGCU---UCgGCAa-CGGCUU--CGGGAGCC- -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 46146 | 0.69 | 0.459206 |
Target: 5'- gUCGAugaccaGGCCGUUGCgCGAAGCggcagCUUCGa -3' miRNA: 3'- gAGCU------UCGGCAACG-GCUUCG-----GGAGCc -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 22980 | 0.66 | 0.660715 |
Target: 5'- aUCGuGGacaUGcUGCUGcAGGCCCUCGGc -3' miRNA: 3'- gAGCuUCg--GCaACGGC-UUCGGGAGCC- -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 8558 | 0.66 | 0.627961 |
Target: 5'- uUCGAgcuggcuccgcuGGaCCGggGCCaGAAGaucaCCCUCGGg -3' miRNA: 3'- gAGCU------------UC-GGCaaCGG-CUUC----GGGAGCC- -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 34875 | 0.67 | 0.562789 |
Target: 5'- --aGAGGCCGUgccGCCGAAcGUCUUCa- -3' miRNA: 3'- gagCUUCGGCAa--CGGCUU-CGGGAGcc -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 5738 | 0.7 | 0.393049 |
Target: 5'- cCUCGAugaccGCCGacGCCGGuacaaccuGGCCCUCGc -3' miRNA: 3'- -GAGCUu----CGGCaaCGGCU--------UCGGGAGCc -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 29355 | 0.72 | 0.332598 |
Target: 5'- uUCGggGUCGgUGCCGAucAGCaccggacccaucuCCUCGGc -3' miRNA: 3'- gAGCuuCGGCaACGGCU--UCG-------------GGAGCC- -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 28991 | 0.71 | 0.358129 |
Target: 5'- aCUCGAaguagaAGCCGggagGCCGA--CCUUCGGg -3' miRNA: 3'- -GAGCU------UCGGCaa--CGGCUucGGGAGCC- -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 9274 | 0.66 | 0.627961 |
Target: 5'- -aCGAGGCUGagcGCCGAccagaGGCCaUCGGc -3' miRNA: 3'- gaGCUUCGGCaa-CGGCU-----UCGGgAGCC- -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 39015 | 0.66 | 0.64981 |
Target: 5'- gCUCGGcuGCCGagaacUGCUGGAucuuGCCUUCGGc -3' miRNA: 3'- -GAGCUu-CGGCa----ACGGCUU----CGGGAGCC- -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 44068 | 0.66 | 0.636703 |
Target: 5'- -cCGccGCCG-UGCUGGAGCCCgacgagcaugacCGGg -3' miRNA: 3'- gaGCuuCGGCaACGGCUUCGGGa-----------GCC- -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 24585 | 0.69 | 0.453301 |
Target: 5'- gUCGAacgcuggccacaccuGGUCGUUGCCGAGGCugaCCUUcaGGa -3' miRNA: 3'- gAGCU---------------UCGGCAACGGCUUCG---GGAG--CC- -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 19474 | 0.69 | 0.469137 |
Target: 5'- -cCGAugGGCCGgcggcUGCCGGuggaccGCUCUCGGa -3' miRNA: 3'- gaGCU--UCGGCa----ACGGCUu-----CGGGAGCC- -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 46040 | 0.68 | 0.499563 |
Target: 5'- -gCGGAcCCGUcGCCGGucGCCUUCGGu -3' miRNA: 3'- gaGCUUcGGCAaCGGCUu-CGGGAGCC- -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 25256 | 0.68 | 0.541419 |
Target: 5'- -cCGAuAGCCGa--CCGGAGCCCgucgUCGGa -3' miRNA: 3'- gaGCU-UCGGCaacGGCUUCGGG----AGCC- -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 47328 | 0.66 | 0.627961 |
Target: 5'- gCUUGGugcuGCCGUacaUGCUcauGCCCUUGGg -3' miRNA: 3'- -GAGCUu---CGGCA---ACGGcuuCGGGAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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