Results 1 - 20 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7187 | 3' | -56.3 | NC_001900.1 | + | 5738 | 0.7 | 0.393049 |
Target: 5'- cCUCGAugaccGCCGacGCCGGuacaaccuGGCCCUCGc -3' miRNA: 3'- -GAGCUu----CGGCaaCGGCU--------UCGGGAGCc -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 8558 | 0.66 | 0.627961 |
Target: 5'- uUCGAgcuggcuccgcuGGaCCGggGCCaGAAGaucaCCCUCGGg -3' miRNA: 3'- gAGCU------------UC-GGCaaCGG-CUUC----GGGAGCC- -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 9274 | 0.66 | 0.627961 |
Target: 5'- -aCGAGGCUGagcGCCGAccagaGGCCaUCGGc -3' miRNA: 3'- gaGCUUCGGCaa-CGGCU-----UCGGgAGCC- -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 13821 | 0.66 | 0.627961 |
Target: 5'- gCUgGGugAGCgGgugGaCCGAgaAGCCCUCGGa -3' miRNA: 3'- -GAgCU--UCGgCaa-C-GGCU--UCGGGAGCC- -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 15276 | 0.66 | 0.638888 |
Target: 5'- gUCGAGGCCGUgGCCGcacGGU--UCGGc -3' miRNA: 3'- gAGCUUCGGCAaCGGCu--UCGggAGCC- -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 15819 | 0.76 | 0.185194 |
Target: 5'- -gCGAAGCCGUgGUCGAGGCCgUCa- -3' miRNA: 3'- gaGCUUCGGCAaCGGCUUCGGgAGcc -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 17715 | 0.66 | 0.617037 |
Target: 5'- gCUCuGggGCCugUGCUGAccguggucgcugAGaCCCUCGGc -3' miRNA: 3'- -GAG-CuuCGGcaACGGCU------------UC-GGGAGCC- -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 19474 | 0.69 | 0.469137 |
Target: 5'- -cCGAugGGCCGgcggcUGCCGGuggaccGCUCUCGGa -3' miRNA: 3'- gaGCU--UCGGCa----ACGGCUu-----CGGGAGCC- -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 20006 | 0.66 | 0.638888 |
Target: 5'- -cCGAGGCCGUggucgcgcUGCUGggG-UCUCGa -3' miRNA: 3'- gaGCUUCGGCA--------ACGGCuuCgGGAGCc -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 22980 | 0.66 | 0.660715 |
Target: 5'- aUCGuGGacaUGcUGCUGcAGGCCCUCGGc -3' miRNA: 3'- gAGCuUCg--GCaACGGC-UUCGGGAGCC- -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 24585 | 0.69 | 0.453301 |
Target: 5'- gUCGAacgcuggccacaccuGGUCGUUGCCGAGGCugaCCUUcaGGa -3' miRNA: 3'- gAGCU---------------UCGGCAACGGCUUCG---GGAG--CC- -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 25256 | 0.68 | 0.541419 |
Target: 5'- -cCGAuAGCCGa--CCGGAGCCCgucgUCGGa -3' miRNA: 3'- gaGCU-UCGGCaacGGCUUCGGG----AGCC- -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 25749 | 0.66 | 0.617037 |
Target: 5'- aCUCGGcccAGCCGggGCCGAccGCCg-UGGu -3' miRNA: 3'- -GAGCU---UCGGCaaCGGCUu-CGGgaGCC- -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 26066 | 0.66 | 0.638888 |
Target: 5'- cCUUGggGuuGaUGCCGAGGgCCUg-- -3' miRNA: 3'- -GAGCuuCggCaACGGCUUCgGGAgcc -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 27996 | 0.67 | 0.57356 |
Target: 5'- uUCGAAcaCGacgUG-CGGAGCCCUCGGg -3' miRNA: 3'- gAGCUUcgGCa--ACgGCUUCGGGAGCC- -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 28991 | 0.71 | 0.358129 |
Target: 5'- aCUCGAaguagaAGCCGggagGCCGA--CCUUCGGg -3' miRNA: 3'- -GAGCU------UCGGCaa--CGGCUucGGGAGCC- -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 29355 | 0.72 | 0.332598 |
Target: 5'- uUCGggGUCGgUGCCGAucAGCaccggacccaucuCCUCGGc -3' miRNA: 3'- gAGCuuCGGCaACGGCU--UCG-------------GGAGCC- -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 30619 | 1.1 | 0.000703 |
Target: 5'- cCUCGAAGCCGUUGCCGAAGCCCUCGGc -3' miRNA: 3'- -GAGCUUCGGCAACGGCUUCGGGAGCC- -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 31846 | 0.7 | 0.430106 |
Target: 5'- gUCGAGGauGUucuggagcUGCUGGAGCCC-CGGa -3' miRNA: 3'- gAGCUUCggCA--------ACGGCUUCGGGaGCC- -5' |
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7187 | 3' | -56.3 | NC_001900.1 | + | 33915 | 0.7 | 0.393049 |
Target: 5'- cCUCGAAGCCGaacuCgGGAGCgugCCUCGGc -3' miRNA: 3'- -GAGCUUCGGCaac-GgCUUCG---GGAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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