Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7189 | 5' | -54.7 | NC_001900.1 | + | 38537 | 0.66 | 0.721207 |
Target: 5'- -gCCugGGACCg-UCGGGUCggcGUCGAc -3' miRNA: 3'- ggGGugUCUGGgaAGUCCAG---UAGCUu -5' |
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7189 | 5' | -54.7 | NC_001900.1 | + | 23954 | 0.66 | 0.688388 |
Target: 5'- aCCCACGGACCa-UCGGGUgucUGUUGGc -3' miRNA: 3'- gGGGUGUCUGGgaAGUCCA---GUAGCUu -5' |
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7189 | 5' | -54.7 | NC_001900.1 | + | 33605 | 0.66 | 0.688388 |
Target: 5'- aCCgGCAGugCggcaAGGUCAUCGAu -3' miRNA: 3'- gGGgUGUCugGgaagUCCAGUAGCUu -5' |
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7189 | 5' | -54.7 | NC_001900.1 | + | 37589 | 0.66 | 0.688388 |
Target: 5'- cCCCCACAGGCgCUcguuguagUCGGGguaCcgCGAc -3' miRNA: 3'- -GGGGUGUCUGgGA--------AGUCCa--GuaGCUu -5' |
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7189 | 5' | -54.7 | NC_001900.1 | + | 39576 | 0.67 | 0.632725 |
Target: 5'- uCCCCAUcGcucCCCUUCGGGUUGUCc-- -3' miRNA: 3'- -GGGGUGuCu--GGGAAGUCCAGUAGcuu -5' |
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7189 | 5' | -54.7 | NC_001900.1 | + | 5065 | 0.67 | 0.643901 |
Target: 5'- uCCCCGguGGCCCggugaAGGUCucgcaacgaggcGUCGAc -3' miRNA: 3'- -GGGGUguCUGGGaag--UCCAG------------UAGCUu -5' |
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7189 | 5' | -54.7 | NC_001900.1 | + | 25492 | 0.68 | 0.577031 |
Target: 5'- cUCCCACGG-CCCgg-AGGUCGUgGAu -3' miRNA: 3'- -GGGGUGUCuGGGaagUCCAGUAgCUu -5' |
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7189 | 5' | -54.7 | NC_001900.1 | + | 45568 | 0.69 | 0.533257 |
Target: 5'- aCCgGcCAGACCCUgCGGGUUcUCGGAc -3' miRNA: 3'- gGGgU-GUCUGGGAaGUCCAGuAGCUU- -5' |
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7189 | 5' | -54.7 | NC_001900.1 | + | 3066 | 0.69 | 0.539755 |
Target: 5'- aCCCGCcGACCCgaccggcgggaAGGUCGUUGAc -3' miRNA: 3'- gGGGUGuCUGGGaag--------UCCAGUAGCUu -5' |
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7189 | 5' | -54.7 | NC_001900.1 | + | 42720 | 0.69 | 0.544102 |
Target: 5'- gCUCACAGACCCUccUCGauGUCGUUGAc -3' miRNA: 3'- gGGGUGUCUGGGA--AGUc-CAGUAGCUu -5' |
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7189 | 5' | -54.7 | NC_001900.1 | + | 9932 | 0.7 | 0.439908 |
Target: 5'- gCCCC-CGG-CCgUcCAGGUCAUCGGGg -3' miRNA: 3'- -GGGGuGUCuGGgAaGUCCAGUAGCUU- -5' |
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7189 | 5' | -54.7 | NC_001900.1 | + | 28263 | 1.08 | 0.001213 |
Target: 5'- gCCCCACAGACCCUUCAGGUCAUCGAAc -3' miRNA: 3'- -GGGGUGUCUGGGAAGUCCAGUAGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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