Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7190 | 3' | -49.8 | NC_001900.1 | + | 42802 | 0.72 | 0.664835 |
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Target: 5'- --cGUGGaUCugCCGUGggUugGUCa- -3' miRNA: 3'- gcaCAUC-AGugGGCACuuAugCAGcu -5' |
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| 7190 | 3' | -49.8 | NC_001900.1 | + | 32243 | 0.71 | 0.741559 |
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Target: 5'- cCG-GUGGUUGCCCGUGAcgaccaucggcgaGUugGUCa- -3' miRNA: 3'- -GCaCAUCAGUGGGCACU-------------UAugCAGcu -5' |
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| 7190 | 3' | -49.8 | NC_001900.1 | + | 5588 | 0.68 | 0.884464 |
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Target: 5'- --cGUGGuUCACCCGcUGGAgcUGUCGGa -3' miRNA: 3'- gcaCAUC-AGUGGGC-ACUUauGCAGCU- -5' |
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| 7190 | 3' | -49.8 | NC_001900.1 | + | 34903 | 0.69 | 0.860123 |
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Target: 5'- uCGUGUAGUCgACCCGcGccAGUGCGggGAu -3' miRNA: 3'- -GCACAUCAG-UGGGCaC--UUAUGCagCU- -5' |
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| 7190 | 3' | -49.8 | NC_001900.1 | + | 32756 | 0.72 | 0.709759 |
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Target: 5'- -aUGUGGUUGCCCGccUGGAcUGCGUCGu -3' miRNA: 3'- gcACAUCAGUGGGC--ACUU-AUGCAGCu -5' |
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| 7190 | 3' | -49.8 | NC_001900.1 | + | 28064 | 1.1 | 0.003163 |
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Target: 5'- aCGUGUAGUCACCCGUGAAUACGUCGAc -3' miRNA: 3'- -GCACAUCAGUGGGCACUUAUGCAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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