Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7191 | 3' | -61.9 | NC_001900.1 | + | 3175 | 0.7 | 0.18739 |
Target: 5'- cGGUUGcCGCUACCGACUGCucccccGCCGGGgGu -3' miRNA: 3'- -CUAGC-GCGGUGGCUGGCG------CGGCUCgU- -5' |
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7191 | 3' | -61.9 | NC_001900.1 | + | 26036 | 0.71 | 0.164386 |
Target: 5'- --gCGUGCCGCCGugC-CGUCGGGUAc -3' miRNA: 3'- cuaGCGCGGUGGCugGcGCGGCUCGU- -5' |
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7191 | 3' | -61.9 | NC_001900.1 | + | 2449 | 0.73 | 0.119237 |
Target: 5'- aGGUCGacgGCC-CCGGCUGCGuCCGGGCu -3' miRNA: 3'- -CUAGCg--CGGuGGCUGGCGC-GGCUCGu -5' |
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7191 | 3' | -61.9 | NC_001900.1 | + | 23317 | 0.73 | 0.119237 |
Target: 5'- cGGUCG-GCC-CCGGCUGgGCCGAGUu -3' miRNA: 3'- -CUAGCgCGGuGGCUGGCgCGGCUCGu -5' |
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7191 | 3' | -61.9 | NC_001900.1 | + | 16895 | 0.75 | 0.079014 |
Target: 5'- gGAUCGuCGCCAUCG-CCGCGCCGcuugucGGCu -3' miRNA: 3'- -CUAGC-GCGGUGGCuGGCGCGGC------UCGu -5' |
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7191 | 3' | -61.9 | NC_001900.1 | + | 31201 | 0.66 | 0.355866 |
Target: 5'- cGAUUGgGCCGacgaccuccuucUUGGCCGUGCCGucAGCGa -3' miRNA: 3'- -CUAGCgCGGU------------GGCUGGCGCGGC--UCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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