Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7191 | 3' | -61.9 | NC_001900.1 | + | 44907 | 0.67 | 0.31962 |
Target: 5'- --aCGCGaguuguuuggggucaCCGCCGGCCGCuucGCCGgucGGCAg -3' miRNA: 3'- cuaGCGC---------------GGUGGCUGGCG---CGGC---UCGU- -5' |
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7191 | 3' | -61.9 | NC_001900.1 | + | 37518 | 0.67 | 0.324186 |
Target: 5'- gGGUCGgGUCGCCGGCCggaGUGCCuucGGUAg -3' miRNA: 3'- -CUAGCgCGGUGGCUGG---CGCGGc--UCGU- -5' |
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7191 | 3' | -61.9 | NC_001900.1 | + | 24551 | 0.66 | 0.339758 |
Target: 5'- --aCGCGCCgACCG-CCGUGaCCGgagucAGCAc -3' miRNA: 3'- cuaGCGCGG-UGGCuGGCGC-GGC-----UCGU- -5' |
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7191 | 3' | -61.9 | NC_001900.1 | + | 25756 | 0.66 | 0.347746 |
Target: 5'- --cCGCGCC-CCGGCgCaCGCgGAGCu -3' miRNA: 3'- cuaGCGCGGuGGCUG-GcGCGgCUCGu -5' |
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7191 | 3' | -61.9 | NC_001900.1 | + | 26978 | 0.66 | 0.355866 |
Target: 5'- uGUCGUGCCGCUGGCCucggaagGCCG-GCu -3' miRNA: 3'- cUAGCGCGGUGGCUGGcg-----CGGCuCGu -5' |
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7191 | 3' | -61.9 | NC_001900.1 | + | 31201 | 0.66 | 0.355866 |
Target: 5'- cGAUUGgGCCGacgaccuccuucUUGGCCGUGCCGucAGCGa -3' miRNA: 3'- -CUAGCgCGGU------------GGCUGGCGCGGC--UCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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