Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7191 | 3' | -61.9 | NC_001900.1 | + | 2449 | 0.73 | 0.119237 |
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Target: 5'- aGGUCGacgGCC-CCGGCUGCGuCCGGGCu -3' miRNA: 3'- -CUAGCg--CGGuGGCUGGCGC-GGCUCGu -5' |
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| 7191 | 3' | -61.9 | NC_001900.1 | + | 23317 | 0.73 | 0.119237 |
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Target: 5'- cGGUCG-GCC-CCGGCUGgGCCGAGUu -3' miRNA: 3'- -CUAGCgCGGuGGCUGGCgCGGCUCGu -5' |
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| 7191 | 3' | -61.9 | NC_001900.1 | + | 19157 | 0.73 | 0.109902 |
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Target: 5'- -uUCGCGUC-CUGGCCGCGCucuCGGGCGa -3' miRNA: 3'- cuAGCGCGGuGGCUGGCGCG---GCUCGU- -5' |
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| 7191 | 3' | -61.9 | NC_001900.1 | + | 16895 | 0.75 | 0.079014 |
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Target: 5'- gGAUCGuCGCCAUCG-CCGCGCCGcuugucGGCu -3' miRNA: 3'- -CUAGC-GCGGUGGCuGGCGCGGC------UCGu -5' |
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| 7191 | 3' | -61.9 | NC_001900.1 | + | 39288 | 0.76 | 0.068759 |
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Target: 5'- --gCGgGaCCACCggGACCGCGCCGAGCc -3' miRNA: 3'- cuaGCgC-GGUGG--CUGGCGCGGCUCGu -5' |
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| 7191 | 3' | -61.9 | NC_001900.1 | + | 27801 | 1.07 | 0.000262 |
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Target: 5'- cGAUCGCGCCACCGACCGCGCCGAGCAc -3' miRNA: 3'- -CUAGCGCGGUGGCUGGCGCGGCUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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