Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7192 | 3' | -56.8 | NC_001900.1 | + | 7316 | 0.66 | 0.625553 |
Target: 5'- --cUGGGCUUGGUCGGGUGuaccuccgacGCCGAcGUg -3' miRNA: 3'- ugaAUCCGAGCUGGCCCAU----------CGGCU-CG- -5' |
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7192 | 3' | -56.8 | NC_001900.1 | + | 42077 | 0.66 | 0.572001 |
Target: 5'- ---gGGGCagCGACCGGGUGGauccacucgucagagUGGGCa -3' miRNA: 3'- ugaaUCCGa-GCUGGCCCAUCg--------------GCUCG- -5' |
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7192 | 3' | -56.8 | NC_001900.1 | + | 40275 | 0.66 | 0.569833 |
Target: 5'- cACgcGGGUcauguccUCGACCGuGUGGCCGgucGGCa -3' miRNA: 3'- -UGaaUCCG-------AGCUGGCcCAUCGGC---UCG- -5' |
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7192 | 3' | -56.8 | NC_001900.1 | + | 23679 | 0.66 | 0.624455 |
Target: 5'- cACUUcgucGGCUCGGCguucaacugcuccUGGGgAGCCGGGa -3' miRNA: 3'- -UGAAu---CCGAGCUG-------------GCCCaUCGGCUCg -5' |
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7192 | 3' | -56.8 | NC_001900.1 | + | 20649 | 0.67 | 0.560105 |
Target: 5'- cACggAGGCcaaauccCGcuACUGGGUGGUCGAGUu -3' miRNA: 3'- -UGaaUCCGa------GC--UGGCCCAUCGGCUCG- -5' |
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7192 | 3' | -56.8 | NC_001900.1 | + | 19318 | 0.67 | 0.507053 |
Target: 5'- uACggcGGUggGGCCaacacGGUGGCCGAGCa -3' miRNA: 3'- -UGaauCCGagCUGGc----CCAUCGGCUCG- -5' |
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7192 | 3' | -56.8 | NC_001900.1 | + | 31601 | 0.67 | 0.560105 |
Target: 5'- aGCUUcuggcGGUUCGGCCGGuucgGGCCGAc- -3' miRNA: 3'- -UGAAu----CCGAGCUGGCCca--UCGGCUcg -5' |
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7192 | 3' | -56.8 | NC_001900.1 | + | 33926 | 0.71 | 0.32172 |
Target: 5'- aACUcGGGagcgugcCUCGGCUGGuGUGGCCGAcGCu -3' miRNA: 3'- -UGAaUCC-------GAGCUGGCC-CAUCGGCU-CG- -5' |
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7192 | 3' | -56.8 | NC_001900.1 | + | 10650 | 0.75 | 0.192907 |
Target: 5'- cACUgcgGGGCUCG-CCGGGUAcGUCcAGCg -3' miRNA: 3'- -UGAa--UCCGAGCuGGCCCAU-CGGcUCG- -5' |
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7192 | 3' | -56.8 | NC_001900.1 | + | 25162 | 0.77 | 0.127993 |
Target: 5'- cGCagAGGCUCgGGCCGGGUacgAGgCGAGCc -3' miRNA: 3'- -UGaaUCCGAG-CUGGCCCA---UCgGCUCG- -5' |
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7192 | 3' | -56.8 | NC_001900.1 | + | 27425 | 0.95 | 0.006123 |
Target: 5'- aACUgauGCUCGACCGGGUAGCCGAGCa -3' miRNA: 3'- -UGAaucCGAGCUGGCCCAUCGGCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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