Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7192 | 5' | -54.7 | NC_001900.1 | + | 14795 | 0.71 | 0.427342 |
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Target: 5'- uGACGCGGUUcCUGAUCCgggcgacguucucAGCGGUcGCu -3' miRNA: 3'- gCUGCGCUAGaGGCUAGG-------------UCGUCA-CG- -5' |
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| 7192 | 5' | -54.7 | NC_001900.1 | + | 3538 | 0.71 | 0.418756 |
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Target: 5'- uCGACGCGGcgCUCgGggCCGGUaacggccuGGUGCa -3' miRNA: 3'- -GCUGCGCUa-GAGgCuaGGUCG--------UCACG- -5' |
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| 7192 | 5' | -54.7 | NC_001900.1 | + | 2016 | 0.72 | 0.40934 |
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Target: 5'- gGGCGCGuGUCaUCCGAgacUUCAGCGacGUGCa -3' miRNA: 3'- gCUGCGC-UAG-AGGCU---AGGUCGU--CACG- -5' |
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| 7192 | 5' | -54.7 | NC_001900.1 | + | 48721 | 0.72 | 0.40934 |
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Target: 5'- aGGCGCGGcgCUa-GAUCCGGCGGUuGCc -3' miRNA: 3'- gCUGCGCUa-GAggCUAGGUCGUCA-CG- -5' |
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| 7192 | 5' | -54.7 | NC_001900.1 | + | 18153 | 0.75 | 0.266687 |
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Target: 5'- uCGGCGCaGUCgccgagugggucggcUCCuGGUCCAGCGGUGUg -3' miRNA: 3'- -GCUGCGcUAG---------------AGG-CUAGGUCGUCACG- -5' |
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| 7192 | 5' | -54.7 | NC_001900.1 | + | 3794 | 0.76 | 0.231797 |
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Target: 5'- aCGACGCGcgugcguUCUCCGuagCCAGCGGaGCc -3' miRNA: 3'- -GCUGCGCu------AGAGGCua-GGUCGUCaCG- -5' |
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| 7192 | 5' | -54.7 | NC_001900.1 | + | 32183 | 0.76 | 0.225774 |
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Target: 5'- uGGCGaCGAUCccgacgCCGAUCCAcGCuGUGCg -3' miRNA: 3'- gCUGC-GCUAGa-----GGCUAGGU-CGuCACG- -5' |
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| 7192 | 5' | -54.7 | NC_001900.1 | + | 48417 | 0.78 | 0.172585 |
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Target: 5'- cCGGCGCGAUCguuUCCGAUCCGccuaucGCGucGUGCu -3' miRNA: 3'- -GCUGCGCUAG---AGGCUAGGU------CGU--CACG- -5' |
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| 7192 | 5' | -54.7 | NC_001900.1 | + | 27461 | 1.11 | 0.000773 |
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Target: 5'- cCGACGCGAUCUCCGAUCCAGCAGUGCc -3' miRNA: 3'- -GCUGCGCUAGAGGCUAGGUCGUCACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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