Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7192 | 5' | -54.7 | NC_001900.1 | + | 40488 | 0.7 | 0.507883 |
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Target: 5'- cCGAgGgUGAUCUucuggccCCGGUCCAGCGGaGCc -3' miRNA: 3'- -GCUgC-GCUAGA-------GGCUAGGUCGUCaCG- -5' |
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| 7192 | 5' | -54.7 | NC_001900.1 | + | 46046 | 0.7 | 0.508932 |
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Target: 5'- cCGuCGcCGGUCgccUUCGGUCagCAGCAGUGCg -3' miRNA: 3'- -GCuGC-GCUAG---AGGCUAG--GUCGUCACG- -5' |
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| 7192 | 5' | -54.7 | NC_001900.1 | + | 41583 | 0.68 | 0.628391 |
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Target: 5'- aGACGCcccauccaagGGUCaUCUuggGAUCCGGCAGaUGCc -3' miRNA: 3'- gCUGCG----------CUAG-AGG---CUAGGUCGUC-ACG- -5' |
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| 7192 | 5' | -54.7 | NC_001900.1 | + | 10587 | 0.67 | 0.661538 |
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Target: 5'- uCGACGCGAUUaCCGc-UgAGCuGUGCg -3' miRNA: 3'- -GCUGCGCUAGaGGCuaGgUCGuCACG- -5' |
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| 7192 | 5' | -54.7 | NC_001900.1 | + | 30984 | 0.67 | 0.661538 |
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Target: 5'- aCGACGCGAUggaCGcgCCAGCc-UGCa -3' miRNA: 3'- -GCUGCGCUAgagGCuaGGUCGucACG- -5' |
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| 7192 | 5' | -54.7 | NC_001900.1 | + | 14234 | 0.66 | 0.704211 |
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Target: 5'- aGAUGCugaccgaGGUCUCCGAgaUCCugAGcCGGUGUg -3' miRNA: 3'- gCUGCG-------CUAGAGGCU--AGG--UC-GUCACG- -5' |
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| 7192 | 5' | -54.7 | NC_001900.1 | + | 18541 | 0.66 | 0.726784 |
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Target: 5'- gGACaCGGUCUUCacggcccgGAUCU-GCAGUGCg -3' miRNA: 3'- gCUGcGCUAGAGG--------CUAGGuCGUCACG- -5' |
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| 7192 | 5' | -54.7 | NC_001900.1 | + | 32018 | 0.66 | 0.726784 |
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Target: 5'- aGACgGCGGUCUuggCCGcuUCCAGCGcggGCa -3' miRNA: 3'- gCUG-CGCUAGA---GGCu-AGGUCGUca-CG- -5' |
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| 7192 | 5' | -54.7 | NC_001900.1 | + | 39719 | 0.66 | 0.737392 |
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Target: 5'- uCGACGUacagccgggGGUaUCCGAcgugagCCAGCAGUGa -3' miRNA: 3'- -GCUGCG---------CUAgAGGCUa-----GGUCGUCACg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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