Results 21 - 29 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7192 | 5' | -54.7 | NC_001900.1 | + | 45972 | 0.69 | 0.572242 |
Target: 5'- uCGACGuUGAUCacUUCGAUCCcuucggcuuggcaGGCGGUGUc -3' miRNA: 3'- -GCUGC-GCUAG--AGGCUAGG-------------UCGUCACG- -5' |
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7192 | 5' | -54.7 | NC_001900.1 | + | 24500 | 0.69 | 0.562434 |
Target: 5'- uCGACcCGGUgcugaCUCCGGUCaCGGCGGUcgGCg -3' miRNA: 3'- -GCUGcGCUA-----GAGGCUAG-GUCGUCA--CG- -5' |
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7192 | 5' | -54.7 | NC_001900.1 | + | 15439 | 0.69 | 0.562434 |
Target: 5'- aCGACGCGAUCgagcugCCGAUCgAugaccuugccGCAcUGCc -3' miRNA: 3'- -GCUGCGCUAGa-----GGCUAGgU----------CGUcACG- -5' |
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7192 | 5' | -54.7 | NC_001900.1 | + | 24572 | 0.69 | 0.551591 |
Target: 5'- uCGACGUGAuUCUCCucgGAgCUGGCGGUGg -3' miRNA: 3'- -GCUGCGCU-AGAGG---CUaGGUCGUCACg -5' |
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7192 | 5' | -54.7 | NC_001900.1 | + | 19847 | 0.69 | 0.54943 |
Target: 5'- cCGACGCGAggagcagaucagCUCCGAgUCgGGCuccccGGUGUg -3' miRNA: 3'- -GCUGCGCUa-----------GAGGCU-AGgUCG-----UCACG- -5' |
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7192 | 5' | -54.7 | NC_001900.1 | + | 1064 | 0.71 | 0.437002 |
Target: 5'- cCGACGCaugggcggaagagGAUCcgCCGGUagCGGCGGUGCc -3' miRNA: 3'- -GCUGCG-------------CUAGa-GGCUAg-GUCGUCACG- -5' |
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7192 | 5' | -54.7 | NC_001900.1 | + | 3538 | 0.71 | 0.418756 |
Target: 5'- uCGACGCGGcgCUCgGggCCGGUaacggccuGGUGCa -3' miRNA: 3'- -GCUGCGCUa-GAGgCuaGGUCG--------UCACG- -5' |
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7192 | 5' | -54.7 | NC_001900.1 | + | 18153 | 0.75 | 0.266687 |
Target: 5'- uCGGCGCaGUCgccgagugggucggcUCCuGGUCCAGCGGUGUg -3' miRNA: 3'- -GCUGCGcUAG---------------AGG-CUAGGUCGUCACG- -5' |
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7192 | 5' | -54.7 | NC_001900.1 | + | 48417 | 0.78 | 0.172585 |
Target: 5'- cCGGCGCGAUCguuUCCGAUCCGccuaucGCGucGUGCu -3' miRNA: 3'- -GCUGCGCUAG---AGGCUAGGU------CGU--CACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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