Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
7193 | 5' | -56.5 | NC_001900.1 | + | 28236 | 0.72 | 0.293653 |
Target: 5'- --gGCCUCGucgguaGCGUCgUGCAGUCGACGGa -3' miRNA: 3'- uagCGGGGC------UGCGG-ACGUUAGUUGCC- -5' |
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7193 | 5' | -56.5 | NC_001900.1 | + | 25538 | 0.75 | 0.17868 |
Target: 5'- -cCGCCCCGGaagagGCCUGCAGccUCAcCGGg -3' miRNA: 3'- uaGCGGGGCUg----CGGACGUU--AGUuGCC- -5' |
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7193 | 5' | -56.5 | NC_001900.1 | + | 27147 | 1.09 | 0.000628 |
Target: 5'- gAUCGCCCCGACGCCUGCAAUCAACGGg -3' miRNA: 3'- -UAGCGGGGCUGCGGACGUUAGUUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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