Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7195 | 5' | -58.8 | NC_001900.1 | + | 38909 | 0.71 | 0.238807 |
Target: 5'- -aGCggaGGCCGGaugUCAGCGGCGGUa- -3' miRNA: 3'- gaCGag-CCGGCCg--AGUUGCUGCCAga -5' |
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7195 | 5' | -58.8 | NC_001900.1 | + | 17465 | 0.73 | 0.179129 |
Target: 5'- cCUGUUCaaCCGGCUCAugGAgucCGGUCUg -3' miRNA: 3'- -GACGAGccGGCCGAGUugCU---GCCAGA- -5' |
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7195 | 5' | -58.8 | NC_001900.1 | + | 21253 | 0.73 | 0.169813 |
Target: 5'- -aGCUC-GCCGuGCUCGGCG-CGGUCg -3' miRNA: 3'- gaCGAGcCGGC-CGAGUUGCuGCCAGa -5' |
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7195 | 5' | -58.8 | NC_001900.1 | + | 25899 | 1.07 | 0.000516 |
Target: 5'- uCUGCUCGGCCGGCUCAACGACGGUCUg -3' miRNA: 3'- -GACGAGCCGGCCGAGUUGCUGCCAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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