miRNA: 3'- -GAcGAGCCGGCCGa-GUUGcu---GCCAGa -5'
miRNA: 3'- gaCGa----GCCGGCCG-AGUuGCUGCC--AGA- -5'
miRNA: 3'- gaCGa-GCCGGCCg----AGUu---GCUGCCAGA- -5'
miRNA: 3'- gaCGAG-CCGGCCGaGUUGCUG---CCaga -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7195 | 5' | -58.8 | NC_001900.1 | + | 1615 | 0.66 | 0.493194 |
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Target: 5'- gCUuCUCGGCCGGUggCGACaccccCGGUCa -3' miRNA: 3'- -GAcGAGCCGGCCGa-GUUGcu---GCCAGa -5' |
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| 7195 | 5' | -58.8 | NC_001900.1 | + | 23588 | 0.66 | 0.513657 |
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Target: 5'- -cGCgccuaCGGUCGGCaUCAcCGACGGggUCUa -3' miRNA: 3'- gaCGa----GCCGGCCG-AGUuGCUGCC--AGA- -5' |
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| 7195 | 5' | -58.8 | NC_001900.1 | + | 26886 | 0.66 | 0.513657 |
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Target: 5'- -cGCaaCGGCCGGaaccgUCGccguCGGCGGUCUg -3' miRNA: 3'- gaCGa-GCCGGCCg----AGUu---GCUGCCAGA- -5' |
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| 7195 | 5' | -58.8 | NC_001900.1 | + | 23608 | 0.66 | 0.513658 |
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Target: 5'- -gGCUCaGGCCGGUgCGACGACcaaGGa-- -3' miRNA: 3'- gaCGAG-CCGGCCGaGUUGCUG---CCaga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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