Results 1 - 20 of 33 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7199 | 3' | -60.7 | NC_001900.1 | + | 48594 | 0.72 | 0.165631 |
Target: 5'- -cCCAcuAGCGGCCGGUaGCCGcgugcauagGCUGCu -3' miRNA: 3'- caGGU--UCGCCGGCCAcUGGCa--------CGGCG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 48541 | 0.66 | 0.402719 |
Target: 5'- uGUCCGGGUGGCUguaaaGGgcacguagGGCaCGUGUgGCa -3' miRNA: 3'- -CAGGUUCGCCGG-----CCa-------CUG-GCACGgCG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 48537 | 0.66 | 0.385956 |
Target: 5'- uUCCAGGCcGCgaGGuUGGCCGgaacguagguugucgGCCGCa -3' miRNA: 3'- cAGGUUCGcCGg-CC-ACUGGCa--------------CGGCG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 46034 | 0.68 | 0.319837 |
Target: 5'- cUCCAGGCGGaCCcGUcGCCG-GUCGCc -3' miRNA: 3'- cAGGUUCGCC-GGcCAcUGGCaCGGCG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 45783 | 0.71 | 0.209585 |
Target: 5'- -aCCAGcggaagucGCGGCCGGUGACCaG-GCagaGCa -3' miRNA: 3'- caGGUU--------CGCCGGCCACUGG-CaCGg--CG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 42850 | 0.67 | 0.367973 |
Target: 5'- -cCCAGGauGCCGGgGACgagaGUGCCGa -3' miRNA: 3'- caGGUUCgcCGGCCaCUGg---CACGGCg -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 42733 | 0.71 | 0.204244 |
Target: 5'- -aCCuugacAGCGGCC-GUGGCCGgugcgGCUGCg -3' miRNA: 3'- caGGu----UCGCCGGcCACUGGCa----CGGCG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 42433 | 0.7 | 0.238123 |
Target: 5'- -cCCGAGgGGaUCGGgcugGcCCGUGCCGUg -3' miRNA: 3'- caGGUUCgCC-GGCCa---CuGGCACGGCG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 41072 | 0.75 | 0.106353 |
Target: 5'- cUCCGAGacguugcgggccaGGCCGGUGACCucgcgGCCGUg -3' miRNA: 3'- cAGGUUCg------------CCGGCCACUGGca---CGGCG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 40294 | 0.69 | 0.27658 |
Target: 5'- -aCCGuGUGGCCGGUcGGCaGUgaGCCGCu -3' miRNA: 3'- caGGUuCGCCGGCCA-CUGgCA--CGGCG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 40128 | 0.66 | 0.410819 |
Target: 5'- uUCCuggccAGCaGCCGcGUccagcagGACCGcUGCCGCg -3' miRNA: 3'- cAGGu----UCGcCGGC-CA-------CUGGC-ACGGCG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 39274 | 0.67 | 0.375609 |
Target: 5'- gGUCCGguucggaAGCGGgaccaCCGG-GACCGcGCCGa -3' miRNA: 3'- -CAGGU-------UCGCC-----GGCCaCUGGCaCGGCg -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 38774 | 0.68 | 0.296879 |
Target: 5'- cUCCAaauacucGGCGGCUGGgcuuCCGUGCagGCu -3' miRNA: 3'- cAGGU-------UCGCCGGCCacu-GGCACGg-CG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 36585 | 0.67 | 0.359614 |
Target: 5'- --aCGGGCuGUCGGUG-CCGUgcaucGCCGCg -3' miRNA: 3'- cagGUUCGcCGGCCACuGGCA-----CGGCG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 34858 | 0.67 | 0.351389 |
Target: 5'- -gCCAgaGGCaccGGCCcagaGGCCGUGCCGCc -3' miRNA: 3'- caGGU--UCG---CCGGcca-CUGGCACGGCG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 34785 | 0.67 | 0.367973 |
Target: 5'- gGUUCGAGCacGGCgGGUGacGCCGUcUCGCu -3' miRNA: 3'- -CAGGUUCG--CCGgCCAC--UGGCAcGGCG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 27448 | 0.66 | 0.402719 |
Target: 5'- cGUUCGAcGCGGaucagcCUGG-GGCCGaGCCGCa -3' miRNA: 3'- -CAGGUU-CGCC------GGCCaCUGGCaCGGCG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 26883 | 0.67 | 0.376464 |
Target: 5'- gGUCgCAA-CGGCCGGa-ACCGUcGCCGUc -3' miRNA: 3'- -CAG-GUUcGCCGGCCacUGGCA-CGGCG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 25679 | 0.71 | 0.193914 |
Target: 5'- -gUgAGGCGGCaCGGUcACCG-GCCGCu -3' miRNA: 3'- caGgUUCGCCG-GCCAcUGGCaCGGCG- -5' |
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7199 | 3' | -60.7 | NC_001900.1 | + | 23379 | 1.09 | 0.000247 |
Target: 5'- gGUCCAAGCGGCCGGUGACCGUGCCGCc -3' miRNA: 3'- -CAGGUUCGCCGGCCACUGGCACGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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