Results 21 - 40 of 40 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7218 | 5' | -56.8 | NC_001900.1 | + | 19264 | 0.66 | 0.656198 |
Target: 5'- aCCAAgGaCGACCCGGAgcggaaguaUGCCaagACGGu -3' miRNA: 3'- cGGUUgCaGCUGGGCCU---------GCGGa--UGCU- -5' |
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7218 | 5' | -56.8 | NC_001900.1 | + | 18766 | 0.66 | 0.612967 |
Target: 5'- gGUCAACG-CGACCgaGGAUGUC-GCGGa -3' miRNA: 3'- -CGGUUGCaGCUGGg-CCUGCGGaUGCU- -5' |
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7218 | 5' | -56.8 | NC_001900.1 | + | 16731 | 0.7 | 0.391755 |
Target: 5'- uGUCAACGUCGACaaGGACaCCaaggGCGGc -3' miRNA: 3'- -CGGUUGCAGCUGggCCUGcGGa---UGCU- -5' |
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7218 | 5' | -56.8 | NC_001900.1 | + | 15850 | 0.75 | 0.214347 |
Target: 5'- cGCCAGCGucacggaggcucuggUCGACCUGGGCGUUgaucccgACGAg -3' miRNA: 3'- -CGGUUGC---------------AGCUGGGCCUGCGGa------UGCU- -5' |
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7218 | 5' | -56.8 | NC_001900.1 | + | 15413 | 1.11 | 0.000593 |
Target: 5'- gGCCAACGUCGACCCGGACGCCUACGAg -3' miRNA: 3'- -CGGUUGCAGCUGGGCCUGCGGAUGCU- -5' |
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7218 | 5' | -56.8 | NC_001900.1 | + | 15291 | 0.66 | 0.645399 |
Target: 5'- gGCCuguACuaCGGCCCGaACGCCUccACGAc -3' miRNA: 3'- -CGGu--UGcaGCUGGGCcUGCGGA--UGCU- -5' |
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7218 | 5' | -56.8 | NC_001900.1 | + | 15087 | 0.66 | 0.623773 |
Target: 5'- uCCGAccCGUCGACCUGGACuggaGCCacCGGc -3' miRNA: 3'- cGGUU--GCAGCUGGGCCUG----CGGauGCU- -5' |
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7218 | 5' | -56.8 | NC_001900.1 | + | 14632 | 0.66 | 0.666977 |
Target: 5'- uCCAACGUCGgugaGCCCaacgaGGuCGUCUGCa- -3' miRNA: 3'- cGGUUGCAGC----UGGG-----CCuGCGGAUGcu -5' |
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7218 | 5' | -56.8 | NC_001900.1 | + | 13374 | 0.67 | 0.580678 |
Target: 5'- uCCGGCGUCGGaucccagaUCUGGACGCCa---- -3' miRNA: 3'- cGGUUGCAGCU--------GGGCCUGCGGaugcu -5' |
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7218 | 5' | -56.8 | NC_001900.1 | + | 12869 | 0.71 | 0.348981 |
Target: 5'- -aCGGCGUCGuCCCGGAgagggguucggcUGCCgagGCGAu -3' miRNA: 3'- cgGUUGCAGCuGGGCCU------------GCGGa--UGCU- -5' |
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7218 | 5' | -56.8 | NC_001900.1 | + | 11555 | 0.66 | 0.623773 |
Target: 5'- gGCCGGCGaccCGAcCCCGGugGUCacUGAc -3' miRNA: 3'- -CGGUUGCa--GCU-GGGCCugCGGauGCU- -5' |
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7218 | 5' | -56.8 | NC_001900.1 | + | 10779 | 0.69 | 0.437799 |
Target: 5'- uGCCGACcUgGGCCgGGACuUCUACGAc -3' miRNA: 3'- -CGGUUGcAgCUGGgCCUGcGGAUGCU- -5' |
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7218 | 5' | -56.8 | NC_001900.1 | + | 8682 | 0.67 | 0.548759 |
Target: 5'- cGCCAagGCGUacgaggagaugcUGACCCGGcuCGCCgagcACGGg -3' miRNA: 3'- -CGGU--UGCA------------GCUGGGCCu-GCGGa---UGCU- -5' |
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7218 | 5' | -56.8 | NC_001900.1 | + | 7911 | 0.66 | 0.656198 |
Target: 5'- cGCacaaGGCGaUCGACCCGGAgaGCCcggaucCGAa -3' miRNA: 3'- -CGg---UUGC-AGCUGGGCCUg-CGGau----GCU- -5' |
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7218 | 5' | -56.8 | NC_001900.1 | + | 6282 | 0.75 | 0.191012 |
Target: 5'- uCC-ACGUCGGgCUGGACGCCUggcACGAc -3' miRNA: 3'- cGGuUGCAGCUgGGCCUGCGGA---UGCU- -5' |
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7218 | 5' | -56.8 | NC_001900.1 | + | 5399 | 0.7 | 0.419005 |
Target: 5'- gGCCAcuGCGgaguaCG-CCagcggGGACGCCUACGAc -3' miRNA: 3'- -CGGU--UGCa----GCuGGg----CCUGCGGAUGCU- -5' |
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7218 | 5' | -56.8 | NC_001900.1 | + | 3875 | 0.72 | 0.302193 |
Target: 5'- cGUCAACGUCGACuucuacuggaaCCGGA--CCUACGAc -3' miRNA: 3'- -CGGUUGCAGCUG-----------GGCCUgcGGAUGCU- -5' |
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7218 | 5' | -56.8 | NC_001900.1 | + | 2386 | 0.67 | 0.559344 |
Target: 5'- uGUgGAUGgccaACCUGGACGCCUACa- -3' miRNA: 3'- -CGgUUGCagc-UGGGCCUGCGGAUGcu -5' |
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7218 | 5' | -56.8 | NC_001900.1 | + | 1813 | 0.66 | 0.663746 |
Target: 5'- cGCUGAUGUCGAgcgacagcguguucCCCGGAaaGCCgggugACGGc -3' miRNA: 3'- -CGGUUGCAGCU--------------GGGCCUg-CGGa----UGCU- -5' |
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7218 | 5' | -56.8 | NC_001900.1 | + | 532 | 0.69 | 0.447376 |
Target: 5'- gGCCAACcucgCGGCCUGGAacaUGCC-GCGAc -3' miRNA: 3'- -CGGUUGca--GCUGGGCCU---GCGGaUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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