Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7224 | 5' | -56 | NC_001900.1 | + | 39913 | 0.68 | 0.520326 |
Target: 5'- --gGGgagCGGGcugUCAUCGGaCGGCcaccACCCg -3' miRNA: 3'- acaCCa--GCCCa--AGUAGCC-GCUG----UGGG- -5' |
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7224 | 5' | -56 | NC_001900.1 | + | 41261 | 0.7 | 0.449387 |
Target: 5'- aGUGGUCaucuGGGUguUCcuuUCGGUGGCugUCa -3' miRNA: 3'- aCACCAG----CCCA--AGu--AGCCGCUGugGG- -5' |
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7224 | 5' | -56 | NC_001900.1 | + | 46006 | 0.66 | 0.671595 |
Target: 5'- --cGGUCGGG----UCGGCGgguGCCCg -3' miRNA: 3'- acaCCAGCCCaaguAGCCGCug-UGGG- -5' |
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7224 | 5' | -56 | NC_001900.1 | + | 46592 | 0.69 | 0.479176 |
Target: 5'- uUGUGGUCua---CGUCGGUGGCAgCCCg -3' miRNA: 3'- -ACACCAGcccaaGUAGCCGCUGU-GGG- -5' |
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7224 | 5' | -56 | NC_001900.1 | + | 46623 | 0.68 | 0.556352 |
Target: 5'- cGUGGUa-GGUccaguccgcgaugucUCGUCGGCcGAC-CCCg -3' miRNA: 3'- aCACCAgcCCA---------------AGUAGCCG-CUGuGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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