Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7224 | 5' | -56 | NC_001900.1 | + | 4936 | 0.67 | 0.595237 |
Target: 5'- ----cUCGGGUUCA--GGCGACuCCCu -3' miRNA: 3'- acaccAGCCCAAGUagCCGCUGuGGG- -5' |
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7224 | 5' | -56 | NC_001900.1 | + | 16987 | 0.67 | 0.606126 |
Target: 5'- gUGUGGUCGcacucggcauGGacggCAUCaaGGCGGCAgCCg -3' miRNA: 3'- -ACACCAGC----------CCaa--GUAG--CCGCUGUgGG- -5' |
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7224 | 5' | -56 | NC_001900.1 | + | 35230 | 0.67 | 0.617037 |
Target: 5'- aGUGGUCcgaGGGcUUC-UCGGUc-CACCCg -3' miRNA: 3'- aCACCAG---CCC-AAGuAGCCGcuGUGGG- -5' |
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7224 | 5' | -56 | NC_001900.1 | + | 11668 | 0.66 | 0.671595 |
Target: 5'- --aGGUCGuccaCAUCGGCGACcugauggacuACCCg -3' miRNA: 3'- acaCCAGCccaaGUAGCCGCUG----------UGGG- -5' |
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7224 | 5' | -56 | NC_001900.1 | + | 46006 | 0.66 | 0.671595 |
Target: 5'- --cGGUCGGG----UCGGCGgguGCCCg -3' miRNA: 3'- acaCCAGCCCaaguAGCCGCug-UGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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