miRNA: 3'- aCACCag---CCCAAGUAGCCg-CU---GUGGG- -5'
miRNA: 3'- acaCCAGCCcAAGua------GCCGCUGUGGG- -5'
miRNA: 3'- acACCAgC-CCAAGUaGCCGCUGUGGG- -5'
miRNA: 3'- -ACACCAG---------CCCaaGUAG------CCGCUGUGGG- -5'
miRNA: 3'- -ACACCAGCCCAAGUAGCCGCUGUGGG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7224 | 5' | -56 | NC_001900.1 | + | 11713 | 0.73 | 0.309947 |
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Target: 5'- gGUGGaccaaGGGUUCGUCGGagGAguuCGCCCa -3' miRNA: 3'- aCACCag---CCCAAGUAGCCg-CU---GUGGG- -5' |
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| 7224 | 5' | -56 | NC_001900.1 | + | 1609 | 0.73 | 0.280662 |
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Target: 5'- --aGGUCGGcUUCucggccggUGGCGACACCCc -3' miRNA: 3'- acaCCAGCCcAAGua------GCCGCUGUGGG- -5' |
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| 7224 | 5' | -56 | NC_001900.1 | + | 8303 | 0.76 | 0.195345 |
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Target: 5'- -cUGGUgGuGGUUCA-CGGCGGCGCUCg -3' miRNA: 3'- acACCAgC-CCAAGUaGCCGCUGUGGG- -5' |
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| 7224 | 5' | -56 | NC_001900.1 | + | 5899 | 0.77 | 0.153254 |
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Target: 5'- aUGUGGUCuggauccugGGGgaCAUCagcucaGGCGGCACCCg -3' miRNA: 3'- -ACACCAG---------CCCaaGUAG------CCGCUGUGGG- -5' |
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| 7224 | 5' | -56 | NC_001900.1 | + | 11632 | 1.12 | 0.000492 |
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Target: 5'- cUGUGGUCGGGUUCAUCGGCGACACCCa -3' miRNA: 3'- -ACACCAGCCCAAGUAGCCGCUGUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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