miRNA: 3'- gCCGCGugGCUCGAC-------CGgCCGGCuAG- -5'
miRNA: 3'- gCCGCGUGGCUCgACCG-GC---------------CGGCUag -5'
miRNA: 3'- gCCgCGUGGCu-CGAccGGCCGgCUAG- -5'
miRNA: 3'- gCCgCGugGC--------------UCGACCGG---CCGGCUAG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7238 | 5' | -63.6 | NC_001900.1 | + | 48647 | 0.66 | 0.271995 |
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Target: 5'- gGGUGCugUGAGCUGcacaaucGCgGGCUG-UCg -3' miRNA: 3'- gCCGCGugGCUCGAC-------CGgCCGGCuAG- -5' |
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| 7238 | 5' | -63.6 | NC_001900.1 | + | 17518 | 0.66 | 0.275318 |
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Target: 5'- aGGCGUACCGGGgUGGCaUGaagcagaaggaucucGCCGAc- -3' miRNA: 3'- gCCGCGUGGCUCgACCG-GC---------------CGGCUag -5' |
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| 7238 | 5' | -63.6 | NC_001900.1 | + | 47979 | 0.66 | 0.286171 |
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Target: 5'- aGGgGCACCGccGCUa-CCGGCgGAUCc -3' miRNA: 3'- gCCgCGUGGCu-CGAccGGCCGgCUAG- -5' |
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| 7238 | 5' | -63.6 | NC_001900.1 | + | 42497 | 0.66 | 0.305246 |
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Target: 5'- aGG-GCugCGugugcgggucgaacGGCUGGCUguaGGCCGGUUg -3' miRNA: 3'- gCCgCGugGC--------------UCGACCGG---CCGGCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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