Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7242 | 5' | -57.1 | NC_001900.1 | + | 23947 | 0.66 | 0.545506 |
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Target: 5'- uCGguGCGGCUcGGUCcaccUCGAaaCCGGCg -3' miRNA: 3'- -GCguUGCCGGaCCAGu---AGCU--GGUCGa -5' |
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| 7242 | 5' | -57.1 | NC_001900.1 | + | 3035 | 0.66 | 0.545506 |
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Target: 5'- gGCGACGgguccGCCUGGagAUCGAgCgGGCa -3' miRNA: 3'- gCGUUGC-----CGGACCagUAGCU-GgUCGa -5' |
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| 7242 | 5' | -57.1 | NC_001900.1 | + | 26885 | 0.66 | 0.56706 |
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Target: 5'- uCGCAACGGCC-GGaacCGUCGccgUCGGCg -3' miRNA: 3'- -GCGUUGCCGGaCCa--GUAGCu--GGUCGa -5' |
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| 7242 | 5' | -57.1 | NC_001900.1 | + | 43312 | 0.66 | 0.56706 |
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Target: 5'- aGCGAgGGCCaGGUUGU--ACCGGCg -3' miRNA: 3'- gCGUUgCCGGaCCAGUAgcUGGUCGa -5' |
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| 7242 | 5' | -57.1 | NC_001900.1 | + | 30603 | 0.66 | 0.588824 |
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Target: 5'- ----uUGGCCuUGGUCGUCuugugcuccuuGGCCAGCUu -3' miRNA: 3'- gcguuGCCGG-ACCAGUAG-----------CUGGUCGA- -5' |
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| 7242 | 5' | -57.1 | NC_001900.1 | + | 32128 | 0.66 | 0.588824 |
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Target: 5'- aGCGACGGCa-GGcCAgccaguaGGCCAGCc -3' miRNA: 3'- gCGUUGCCGgaCCaGUag-----CUGGUCGa -5' |
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| 7242 | 5' | -57.1 | NC_001900.1 | + | 25442 | 0.66 | 0.599765 |
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Target: 5'- gCGCAccauCaGCUccuUGGUCGUCGcACCGGCc -3' miRNA: 3'- -GCGUu---GcCGG---ACCAGUAGC-UGGUCGa -5' |
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| 7242 | 5' | -57.1 | NC_001900.1 | + | 39191 | 0.66 | 0.599765 |
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Target: 5'- aGCAucaggauucgaGCcGCCgcgucGGUCAUCGACCgaAGCUc -3' miRNA: 3'- gCGU-----------UGcCGGa----CCAGUAGCUGG--UCGA- -5' |
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| 7242 | 5' | -57.1 | NC_001900.1 | + | 29870 | 0.66 | 0.599765 |
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Target: 5'- cCGCAcCGuccuugaacGCCUGGaUCAUCGuCCGGUc -3' miRNA: 3'- -GCGUuGC---------CGGACC-AGUAGCuGGUCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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