Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7268 | 3' | -54.1 | NC_001900.1 | + | 40408 | 0.66 | 0.713759 |
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Target: 5'- cUCACCGAgaGCCCgugcagGAACa-CCAGCg- -3' miRNA: 3'- -AGUGGCUg-UGGGa-----CUUGaaGGUCGag -5' |
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| 7268 | 3' | -54.1 | NC_001900.1 | + | 7262 | 0.66 | 0.713759 |
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Target: 5'- aUCAUCGACGCCCUcacguuCUucgCCcGCUCu -3' miRNA: 3'- -AGUGGCUGUGGGAcuu---GAa--GGuCGAG- -5' |
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| 7268 | 3' | -54.1 | NC_001900.1 | + | 3818 | 0.66 | 0.724674 |
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Target: 5'- gCGCCGcgagguCACCUUGAGCUggaAGUUCa -3' miRNA: 3'- aGUGGCu-----GUGGGACUUGAaggUCGAG- -5' |
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| 7268 | 3' | -54.1 | NC_001900.1 | + | 43207 | 0.66 | 0.724674 |
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Target: 5'- -aGgCGGCACCC-GAGCg--CAGCUCg -3' miRNA: 3'- agUgGCUGUGGGaCUUGaagGUCGAG- -5' |
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| 7268 | 3' | -54.1 | NC_001900.1 | + | 32571 | 0.66 | 0.735493 |
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Target: 5'- gUCACaGGCA-CCUGAGCUUCuCGGUg- -3' miRNA: 3'- -AGUGgCUGUgGGACUUGAAG-GUCGag -5' |
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| 7268 | 3' | -54.1 | NC_001900.1 | + | 44356 | 0.66 | 0.735493 |
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Target: 5'- -gACCGGgaACCaaccggGAgacgACUUCCAGCUCg -3' miRNA: 3'- agUGGCUg-UGGga----CU----UGAAGGUCGAG- -5' |
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| 7268 | 3' | -54.1 | NC_001900.1 | + | 39130 | 0.66 | 0.746203 |
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Target: 5'- cCACCGGCAUCgCgGAGgUcaUCCAGCg- -3' miRNA: 3'- aGUGGCUGUGG-GaCUUgA--AGGUCGag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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