Results 1 - 20 of 33 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7275 | 3' | -58.1 | NC_001900.1 | + | 28659 | 0.66 | 0.559036 |
Target: 5'- cGGUGUUgCUC-ACCUGGaaggugaugcccuccUCGGCgauguaccaggGCAGCu -3' miRNA: 3'- -CCGCGA-GAGuUGGACC---------------AGCCG-----------CGUCG- -5' |
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7275 | 3' | -58.1 | NC_001900.1 | + | 29343 | 0.66 | 0.554771 |
Target: 5'- -aCGCUUUcCAACUucggGGUCGGUGCcgaucAGCa -3' miRNA: 3'- ccGCGAGA-GUUGGa---CCAGCCGCG-----UCG- -5' |
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7275 | 3' | -58.1 | NC_001900.1 | + | 18039 | 0.66 | 0.554771 |
Target: 5'- cGGUGgUCcagaucgucaUCAACCUGGcugCGGcCGUGGUg -3' miRNA: 3'- -CCGCgAG----------AGUUGGACCa--GCC-GCGUCG- -5' |
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7275 | 3' | -58.1 | NC_001900.1 | + | 27602 | 0.66 | 0.548393 |
Target: 5'- cGCGCUCgaucccggaugcagCGAUCaGGUUcgagcaGGCGUAGCa -3' miRNA: 3'- cCGCGAGa-------------GUUGGaCCAG------CCGCGUCG- -5' |
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7275 | 3' | -58.1 | NC_001900.1 | + | 1647 | 0.66 | 0.523124 |
Target: 5'- cGCGCUCUguACgaGGa-GGCGUGGUu -3' miRNA: 3'- cCGCGAGAguUGgaCCagCCGCGUCG- -5' |
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7275 | 3' | -58.1 | NC_001900.1 | + | 35820 | 0.66 | 0.523124 |
Target: 5'- cGGCGUgUUCAccCCUGGcuaCGGC-CAGCg -3' miRNA: 3'- -CCGCGaGAGUu-GGACCa--GCCGcGUCG- -5' |
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7275 | 3' | -58.1 | NC_001900.1 | + | 15548 | 0.66 | 0.512727 |
Target: 5'- cGGC-CUCUC-GCCgGG-CGGUGgAGCc -3' miRNA: 3'- -CCGcGAGAGuUGGaCCaGCCGCgUCG- -5' |
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7275 | 3' | -58.1 | NC_001900.1 | + | 147 | 0.66 | 0.502415 |
Target: 5'- uGGUgGCUCaCAgcGCCUGGgcgaGGCGCucuAGCg -3' miRNA: 3'- -CCG-CGAGaGU--UGGACCag--CCGCG---UCG- -5' |
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7275 | 3' | -58.1 | NC_001900.1 | + | 39118 | 0.66 | 0.502415 |
Target: 5'- cGGCGCUaugcgCUCcccgauGACCUGGaCGGCcgGgGGCu -3' miRNA: 3'- -CCGCGA-----GAG------UUGGACCaGCCG--CgUCG- -5' |
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7275 | 3' | -58.1 | NC_001900.1 | + | 3842 | 0.67 | 0.492195 |
Target: 5'- cGGC-UUCUcCGGgCUGaUCGGCGCGGUg -3' miRNA: 3'- -CCGcGAGA-GUUgGACcAGCCGCGUCG- -5' |
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7275 | 3' | -58.1 | NC_001900.1 | + | 24111 | 0.67 | 0.472051 |
Target: 5'- -cCGC-CUCGgcACCgaGGUUGGCGCGGg -3' miRNA: 3'- ccGCGaGAGU--UGGa-CCAGCCGCGUCg -5' |
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7275 | 3' | -58.1 | NC_001900.1 | + | 1458 | 0.67 | 0.459183 |
Target: 5'- uGCGCUUggcauccgacggugaGugCUGGUCGcGgGCGGCg -3' miRNA: 3'- cCGCGAGag-------------UugGACCAGC-CgCGUCG- -5' |
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7275 | 3' | -58.1 | NC_001900.1 | + | 18694 | 0.68 | 0.423622 |
Target: 5'- uGCGUucuUCUCGACCUGGauccgcuugCGcuccugcuccaGCGCAGCc -3' miRNA: 3'- cCGCG---AGAGUUGGACCa--------GC-----------CGCGUCG- -5' |
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7275 | 3' | -58.1 | NC_001900.1 | + | 37167 | 0.68 | 0.423622 |
Target: 5'- --gGUUCUCGAacuugaaCUGGUCGGCGUacucgaccagAGCu -3' miRNA: 3'- ccgCGAGAGUUg------GACCAGCCGCG----------UCG- -5' |
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7275 | 3' | -58.1 | NC_001900.1 | + | 44799 | 0.68 | 0.414297 |
Target: 5'- aGCGCagcggaagCUUGAUCUGcGUcaCGGCGCGGCc -3' miRNA: 3'- cCGCGa-------GAGUUGGAC-CA--GCCGCGUCG- -5' |
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7275 | 3' | -58.1 | NC_001900.1 | + | 3544 | 0.68 | 0.405099 |
Target: 5'- cGGCGCUCggGGCC-GGUaaCGGCcuGguGCa -3' miRNA: 3'- -CCGCGAGagUUGGaCCA--GCCG--CguCG- -5' |
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7275 | 3' | -58.1 | NC_001900.1 | + | 20956 | 0.68 | 0.396032 |
Target: 5'- cGGCGgUCgUCAACCUGGcUGGaGaCGGCc -3' miRNA: 3'- -CCGCgAG-AGUUGGACCaGCCgC-GUCG- -5' |
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7275 | 3' | -58.1 | NC_001900.1 | + | 38713 | 0.68 | 0.396032 |
Target: 5'- cGGUGgUCgcccuggCGuaaCUGGUCGGuUGCAGCg -3' miRNA: 3'- -CCGCgAGa------GUug-GACCAGCC-GCGUCG- -5' |
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7275 | 3' | -58.1 | NC_001900.1 | + | 35074 | 0.69 | 0.387095 |
Target: 5'- -cCGC-CUCGGCCaGGUCaGCGUAGUc -3' miRNA: 3'- ccGCGaGAGUUGGaCCAGcCGCGUCG- -5' |
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7275 | 3' | -58.1 | NC_001900.1 | + | 33180 | 0.69 | 0.378293 |
Target: 5'- uGGCcgaucaCUCgcCAACCUGGgUGGCgGCAGCa -3' miRNA: 3'- -CCGc-----GAGa-GUUGGACCaGCCG-CGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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