Results 1 - 20 of 36 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7275 | 5' | -55.3 | NC_001900.1 | + | 29087 | 0.66 | 0.717889 |
Target: 5'- aCACcGCCGaUGUCcccgAAGGUCGGCCu- -3' miRNA: 3'- gGUGcCGGCgACAG----UUCCAGUUGGuc -5' |
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7275 | 5' | -55.3 | NC_001900.1 | + | 43277 | 0.66 | 0.717889 |
Target: 5'- gCGUGGCCGauguguagGUCgGAGGUgAACCAGa -3' miRNA: 3'- gGUGCCGGCga------CAG-UUCCAgUUGGUC- -5' |
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7275 | 5' | -55.3 | NC_001900.1 | + | 29003 | 0.66 | 0.717889 |
Target: 5'- -gAUGGCCGaCgacGUaAAGGUCAACUGGg -3' miRNA: 3'- ggUGCCGGC-Ga--CAgUUCCAGUUGGUC- -5' |
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7275 | 5' | -55.3 | NC_001900.1 | + | 39723 | 0.66 | 0.707124 |
Target: 5'- gCGCGaugagaucaGCCGCcagcUCcGGGUCAGCCAGc -3' miRNA: 3'- gGUGC---------CGGCGac--AGuUCCAGUUGGUC- -5' |
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7275 | 5' | -55.3 | NC_001900.1 | + | 21312 | 0.66 | 0.704961 |
Target: 5'- gCACGGCgaGCUGgcuguugaugaaCGAGGUCAGCa-- -3' miRNA: 3'- gGUGCCGg-CGACa-----------GUUCCAGUUGguc -5' |
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7275 | 5' | -55.3 | NC_001900.1 | + | 6643 | 0.66 | 0.700628 |
Target: 5'- uCCAgGGCUGCgUGUgcGGGUCGaacggcuggcuguagGCCGGu -3' miRNA: 3'- -GGUgCCGGCG-ACAguUCCAGU---------------UGGUC- -5' |
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7275 | 5' | -55.3 | NC_001900.1 | + | 15172 | 0.66 | 0.696284 |
Target: 5'- aCCAgcCGGUgGCUccaGUCcAGGUCGACgGGu -3' miRNA: 3'- -GGU--GCCGgCGA---CAGuUCCAGUUGgUC- -5' |
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7275 | 5' | -55.3 | NC_001900.1 | + | 47454 | 0.66 | 0.685381 |
Target: 5'- cCCuuCGGUgGCUGUCAA-GUCAGCgGa -3' miRNA: 3'- -GGu-GCCGgCGACAGUUcCAGUUGgUc -5' |
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7275 | 5' | -55.3 | NC_001900.1 | + | 4110 | 0.66 | 0.685381 |
Target: 5'- aCCGCuGCCGCUGgCAGuuGGUucaucugcCGACCGGc -3' miRNA: 3'- -GGUGcCGGCGACaGUU--CCA--------GUUGGUC- -5' |
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7275 | 5' | -55.3 | NC_001900.1 | + | 38110 | 0.66 | 0.685381 |
Target: 5'- gCCuCGGCguugCGC-GUC-AGGUCGACCAc -3' miRNA: 3'- -GGuGCCG----GCGaCAGuUCCAGUUGGUc -5' |
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7275 | 5' | -55.3 | NC_001900.1 | + | 599 | 0.66 | 0.685381 |
Target: 5'- uCCA-GGCCGC----GAGGUUGGCCGGa -3' miRNA: 3'- -GGUgCCGGCGacagUUCCAGUUGGUC- -5' |
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7275 | 5' | -55.3 | NC_001900.1 | + | 39023 | 0.66 | 0.682099 |
Target: 5'- cCCuCGGCCGCaGUCAguuacgaccuccaaGGGUuCAACUg- -3' miRNA: 3'- -GGuGCCGGCGaCAGU--------------UCCA-GUUGGuc -5' |
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7275 | 5' | -55.3 | NC_001900.1 | + | 37328 | 0.67 | 0.652409 |
Target: 5'- aCgACGGCC-CUGUCGgcguccacgaAGG-CAACCAc -3' miRNA: 3'- -GgUGCCGGcGACAGU----------UCCaGUUGGUc -5' |
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7275 | 5' | -55.3 | NC_001900.1 | + | 30570 | 0.67 | 0.652409 |
Target: 5'- uCCG-GGUgGUacagGUCGAuGGUCGACCAGa -3' miRNA: 3'- -GGUgCCGgCGa---CAGUU-CCAGUUGGUC- -5' |
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7275 | 5' | -55.3 | NC_001900.1 | + | 34162 | 0.67 | 0.630316 |
Target: 5'- -gAUGGCCGCUG---AGGUCGACa-- -3' miRNA: 3'- ggUGCCGGCGACaguUCCAGUUGguc -5' |
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7275 | 5' | -55.3 | NC_001900.1 | + | 37591 | 0.67 | 0.629211 |
Target: 5'- cCCACaGgCGCUcguuguaGUCGGGGUaccgCGACCAGa -3' miRNA: 3'- -GGUGcCgGCGA-------CAGUUCCA----GUUGGUC- -5' |
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7275 | 5' | -55.3 | NC_001900.1 | + | 15111 | 0.68 | 0.586224 |
Target: 5'- gCCAcCGGCUG--GUCGAGcGUCGGCCAc -3' miRNA: 3'- -GGU-GCCGGCgaCAGUUC-CAGUUGGUc -5' |
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7275 | 5' | -55.3 | NC_001900.1 | + | 18127 | 0.68 | 0.564374 |
Target: 5'- aCCACGGCCGCaGcCA-GGUUgaugacgaucugGACCAc -3' miRNA: 3'- -GGUGCCGGCGaCaGUuCCAG------------UUGGUc -5' |
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7275 | 5' | -55.3 | NC_001900.1 | + | 16528 | 0.68 | 0.564374 |
Target: 5'- gCACGGaUCuCcGUCAAGGUCAGCCc- -3' miRNA: 3'- gGUGCC-GGcGaCAGUUCCAGUUGGuc -5' |
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7275 | 5' | -55.3 | NC_001900.1 | + | 46242 | 0.68 | 0.542745 |
Target: 5'- gUCACgGGUgGCaGUCAAGGUgAACaCAGg -3' miRNA: 3'- -GGUG-CCGgCGaCAGUUCCAgUUG-GUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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