Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7283 | 5' | -59.2 | NC_001900.1 | + | 6396 | 0.67 | 0.407082 |
Target: 5'- aGCGGCCGuGGcCggugcGGCUG-CGCCg -3' miRNA: 3'- -CGCCGGCuCCuGagga-CCGACaGUGG- -5' |
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7283 | 5' | -59.2 | NC_001900.1 | + | 41475 | 0.67 | 0.434801 |
Target: 5'- gGgGGuuGGGGGCgcagGGC-GUCACCg -3' miRNA: 3'- -CgCCggCUCCUGaggaCCGaCAGUGG- -5' |
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7283 | 5' | -59.2 | NC_001900.1 | + | 40882 | 0.67 | 0.444281 |
Target: 5'- aCGGUguGGGACgcCCUGGCUGacgagcugCACCg -3' miRNA: 3'- cGCCGgcUCCUGa-GGACCGACa-------GUGG- -5' |
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7283 | 5' | -59.2 | NC_001900.1 | + | 29447 | 0.67 | 0.444281 |
Target: 5'- aUGGCCGAGGAgaugggUCCgGuGCUGaucggCACCg -3' miRNA: 3'- cGCCGGCUCCUg-----AGGaC-CGACa----GUGG- -5' |
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7283 | 5' | -59.2 | NC_001900.1 | + | 35910 | 0.66 | 0.463582 |
Target: 5'- cCGaGCCGAGGugUUCccgGaGCUGUucccaCACCa -3' miRNA: 3'- cGC-CGGCUCCugAGGa--C-CGACA-----GUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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