Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7285 | 3' | -52.6 | NC_001900.1 | + | 7183 | 0.65 | 0.830719 |
Target: 5'- uGGCCGGGA-CUCCGugguagcccagcucAUCGAGCuugGCCAGc -3' miRNA: 3'- -UUGGUCUUgGAGGC--------------UAGCUUG---UGGUC- -5' |
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7285 | 3' | -52.6 | NC_001900.1 | + | 22420 | 0.66 | 0.824158 |
Target: 5'- cGACgAGAACCUCCGcgaCGAGauCACCc- -3' miRNA: 3'- -UUGgUCUUGGAGGCua-GCUU--GUGGuc -5' |
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7285 | 3' | -52.6 | NC_001900.1 | + | 15445 | 0.66 | 0.824158 |
Target: 5'- cGAUC-GAGCUgCCGAUCGAugACCu- -3' miRNA: 3'- -UUGGuCUUGGaGGCUAGCUugUGGuc -5' |
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7285 | 3' | -52.6 | NC_001900.1 | + | 33473 | 0.66 | 0.824157 |
Target: 5'- cGCCAGcGCg-CCGGUCG-ACACCGc -3' miRNA: 3'- uUGGUCuUGgaGGCUAGCuUGUGGUc -5' |
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7285 | 3' | -52.6 | NC_001900.1 | + | 3333 | 0.66 | 0.814601 |
Target: 5'- uGACCAGGcagagcAUCUCCaGGUCGAACggguuGCCGa -3' miRNA: 3'- -UUGGUCU------UGGAGG-CUAGCUUG-----UGGUc -5' |
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7285 | 3' | -52.6 | NC_001900.1 | + | 23033 | 0.66 | 0.784751 |
Target: 5'- uGACCGGAucuucgACCUCagcgacgaGAUCGAgugGCugCGGg -3' miRNA: 3'- -UUGGUCU------UGGAGg-------CUAGCU---UGugGUC- -5' |
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7285 | 3' | -52.6 | NC_001900.1 | + | 3087 | 0.67 | 0.774446 |
Target: 5'- cGCCGGucGCCUUCGGUC-AGCAgCAGu -3' miRNA: 3'- uUGGUCu-UGGAGGCUAGcUUGUgGUC- -5' |
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7285 | 3' | -52.6 | NC_001900.1 | + | 18319 | 0.67 | 0.771323 |
Target: 5'- cAGCCAgGAGCCUgCGucAUCGAACcagcucuugaucuuGCCGGg -3' miRNA: 3'- -UUGGU-CUUGGAgGC--UAGCUUG--------------UGGUC- -5' |
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7285 | 3' | -52.6 | NC_001900.1 | + | 11607 | 0.67 | 0.763984 |
Target: 5'- cGGCCGGAguGCCUUCGGuaguugcggugUCGGACAUguGu -3' miRNA: 3'- -UUGGUCU--UGGAGGCU-----------AGCUUGUGguC- -5' |
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7285 | 3' | -52.6 | NC_001900.1 | + | 40573 | 0.67 | 0.763984 |
Target: 5'- uGCCGacuGACCUaCCGAgaggGAACGCCAGg -3' miRNA: 3'- uUGGUc--UUGGA-GGCUag--CUUGUGGUC- -5' |
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7285 | 3' | -52.6 | NC_001900.1 | + | 29355 | 0.67 | 0.753378 |
Target: 5'- -uUCGGggUCggugCCGAUC-AGCACCGGa -3' miRNA: 3'- uuGGUCuuGGa---GGCUAGcUUGUGGUC- -5' |
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7285 | 3' | -52.6 | NC_001900.1 | + | 4962 | 0.67 | 0.742639 |
Target: 5'- gAGCCGGAAgCUCUcaGUCGAGCcguACCGGu -3' miRNA: 3'- -UUGGUCUUgGAGGc-UAGCUUG---UGGUC- -5' |
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7285 | 3' | -52.6 | NC_001900.1 | + | 34689 | 0.67 | 0.731782 |
Target: 5'- cGCCAugcGAGCCgaCCGG-CGAACACCGc -3' miRNA: 3'- uUGGU---CUUGGa-GGCUaGCUUGUGGUc -5' |
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7285 | 3' | -52.6 | NC_001900.1 | + | 13944 | 0.68 | 0.720817 |
Target: 5'- uGACCAGcuCUUCgGGguagaCGAGCACCGGc -3' miRNA: 3'- -UUGGUCuuGGAGgCUa----GCUUGUGGUC- -5' |
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7285 | 3' | -52.6 | NC_001900.1 | + | 15753 | 0.68 | 0.720817 |
Target: 5'- cGAUCGGGAUCUCCGAagaugugaccgUCGAGCugaaGCCGc -3' miRNA: 3'- -UUGGUCUUGGAGGCU-----------AGCUUG----UGGUc -5' |
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7285 | 3' | -52.6 | NC_001900.1 | + | 30416 | 0.68 | 0.709759 |
Target: 5'- gAGCaGGAACCcgcgCCGGUCGAuguaGCugCAGa -3' miRNA: 3'- -UUGgUCUUGGa---GGCUAGCU----UGugGUC- -5' |
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7285 | 3' | -52.6 | NC_001900.1 | + | 36781 | 0.69 | 0.664835 |
Target: 5'- cGACCgAGAaucgGCCUCCGAcgaUCGGA-ACCAGc -3' miRNA: 3'- -UUGG-UCU----UGGAGGCU---AGCUUgUGGUC- -5' |
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7285 | 3' | -52.6 | NC_001900.1 | + | 23873 | 0.69 | 0.664835 |
Target: 5'- cGACCGGAGCCcgUCG-UCGGACcacaggGCCAGu -3' miRNA: 3'- -UUGGUCUUGGa-GGCuAGCUUG------UGGUC- -5' |
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7285 | 3' | -52.6 | NC_001900.1 | + | 30779 | 0.69 | 0.629619 |
Target: 5'- aGGCCAGAGCgacuccauCUugaucccgaagcaCCGGUCGAGCACCuGg -3' miRNA: 3'- -UUGGUCUUG--------GA-------------GGCUAGCUUGUGGuC- -5' |
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7285 | 3' | -52.6 | NC_001900.1 | + | 9851 | 0.7 | 0.596687 |
Target: 5'- -cCCGGAGCUU-CGGUCGAugACCGa -3' miRNA: 3'- uuGGUCUUGGAgGCUAGCUugUGGUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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