Results 1 - 20 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7291 | 3' | -52.7 | NC_001900.1 | + | 34942 | 0.66 | 0.859696 |
Target: 5'- cGUUCGGCGGcacggccucUGGgCCGGUgCCUCuGGCGa -3' miRNA: 3'- -CGAGUUGCC---------ACUgGGCUA-GGAG-UUGC- -5' |
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7291 | 3' | -52.7 | NC_001900.1 | + | 15286 | 0.66 | 0.859696 |
Target: 5'- cGCUCGGCcuGUacuacGGCCCGAacgCCUCcACGa -3' miRNA: 3'- -CGAGUUGc-CA-----CUGGGCUa--GGAGuUGC- -5' |
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7291 | 3' | -52.7 | NC_001900.1 | + | 10314 | 0.66 | 0.850346 |
Target: 5'- uGCUCcgcauggaGACGGUcuggcguGACCCGAgcaCCcCGACGu -3' miRNA: 3'- -CGAG--------UUGCCA-------CUGGGCUa--GGaGUUGC- -5' |
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7291 | 3' | -52.7 | NC_001900.1 | + | 18497 | 0.66 | 0.833531 |
Target: 5'- cCUCGGCcGUGAacugaCCGAU-CUCAGCGa -3' miRNA: 3'- cGAGUUGcCACUg----GGCUAgGAGUUGC- -5' |
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7291 | 3' | -52.7 | NC_001900.1 | + | 21298 | 0.66 | 0.82436 |
Target: 5'- cGCUgGGCGGUcugaugGAUgCGGUCCUgAACc -3' miRNA: 3'- -CGAgUUGCCA------CUGgGCUAGGAgUUGc -5' |
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7291 | 3' | -52.7 | NC_001900.1 | + | 28341 | 0.66 | 0.814981 |
Target: 5'- uCUCcACGGagcccucccgGACCUGAUCCgUCGACu -3' miRNA: 3'- cGAGuUGCCa---------CUGGGCUAGG-AGUUGc -5' |
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7291 | 3' | -52.7 | NC_001900.1 | + | 5549 | 0.66 | 0.814981 |
Target: 5'- gGCUC-ACGGcaggGGCCUGGUCC-CcACGc -3' miRNA: 3'- -CGAGuUGCCa---CUGGGCUAGGaGuUGC- -5' |
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7291 | 3' | -52.7 | NC_001900.1 | + | 41619 | 0.67 | 0.795641 |
Target: 5'- gGCUCGAUGGagcaccGACCCugGAUCUcCAGCa -3' miRNA: 3'- -CGAGUUGCCa-----CUGGG--CUAGGaGUUGc -5' |
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7291 | 3' | -52.7 | NC_001900.1 | + | 28665 | 0.67 | 0.775598 |
Target: 5'- uGCUCAccuggaaGGUGauGCCC--UCCUCGGCGa -3' miRNA: 3'- -CGAGUug-----CCAC--UGGGcuAGGAGUUGC- -5' |
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7291 | 3' | -52.7 | NC_001900.1 | + | 30060 | 0.67 | 0.774579 |
Target: 5'- cGCgccugCAGCGGguugacgaacgugUGGCaCCGGUCCUCGGa- -3' miRNA: 3'- -CGa----GUUGCC-------------ACUG-GGCUAGGAGUUgc -5' |
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7291 | 3' | -52.7 | NC_001900.1 | + | 7105 | 0.67 | 0.765341 |
Target: 5'- uGCUCca-GGUuuucGAgCCGGUCCUCGAgGa -3' miRNA: 3'- -CGAGuugCCA----CUgGGCUAGGAGUUgC- -5' |
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7291 | 3' | -52.7 | NC_001900.1 | + | 42049 | 0.68 | 0.754943 |
Target: 5'- cGCUCcaucgcgagucgGACGGcuucGGCCCGGacUCgCUCAACGu -3' miRNA: 3'- -CGAG------------UUGCCa---CUGGGCU--AG-GAGUUGC- -5' |
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7291 | 3' | -52.7 | NC_001900.1 | + | 33749 | 0.68 | 0.733773 |
Target: 5'- aGCUCGuUGGcUGACUccaCGAugUCCUCGGCGu -3' miRNA: 3'- -CGAGUuGCC-ACUGG---GCU--AGGAGUUGC- -5' |
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7291 | 3' | -52.7 | NC_001900.1 | + | 35059 | 0.68 | 0.723024 |
Target: 5'- gGCUCGACaacUGACCCG--CCUCGGCc -3' miRNA: 3'- -CGAGUUGcc-ACUGGGCuaGGAGUUGc -5' |
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7291 | 3' | -52.7 | NC_001900.1 | + | 26745 | 0.68 | 0.712181 |
Target: 5'- cCUCu-CGGgGAUCCGGUCCUCGGg- -3' miRNA: 3'- cGAGuuGCCaCUGGGCUAGGAGUUgc -5' |
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7291 | 3' | -52.7 | NC_001900.1 | + | 878 | 0.69 | 0.701259 |
Target: 5'- aGCUCGAUGG-GcAUCCGcUCCUCGAa- -3' miRNA: 3'- -CGAGUUGCCaC-UGGGCuAGGAGUUgc -5' |
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7291 | 3' | -52.7 | NC_001900.1 | + | 10253 | 0.69 | 0.701259 |
Target: 5'- gGCUCucuGCGGc--CCUGAUggCCUCAGCGg -3' miRNA: 3'- -CGAGu--UGCCacuGGGCUA--GGAGUUGC- -5' |
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7291 | 3' | -52.7 | NC_001900.1 | + | 19657 | 0.69 | 0.679216 |
Target: 5'- uGCUC-GCGGccGACCuUGAUCCgCAGCGu -3' miRNA: 3'- -CGAGuUGCCa-CUGG-GCUAGGaGUUGC- -5' |
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7291 | 3' | -52.7 | NC_001900.1 | + | 15834 | 0.7 | 0.645834 |
Target: 5'- aGCUCGACGGUcacaucuucggaGAuCCCGAUCgU-GACGg -3' miRNA: 3'- -CGAGUUGCCA------------CU-GGGCUAGgAgUUGC- -5' |
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7291 | 3' | -52.7 | NC_001900.1 | + | 12954 | 0.7 | 0.605645 |
Target: 5'- uCUCGACGGUGcccagguucagggucGCCUGAUCCgUC-ACGu -3' miRNA: 3'- cGAGUUGCCAC---------------UGGGCUAGG-AGuUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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