Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7311 | 3' | -50.6 | NC_001900.1 | + | 24673 | 0.7 | 0.684445 |
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Target: 5'- -uGGCGGAgcccaugCCUUGuccaccACCGCCAGCu -3' miRNA: 3'- cuUCGUCUa------GGAACucu---UGGUGGUCG- -5' |
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| 7311 | 3' | -50.6 | NC_001900.1 | + | 30813 | 0.72 | 0.592021 |
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Target: 5'- --cGCAGAUCCUggcgaagcUGGuGGACCcCCGGCc -3' miRNA: 3'- cuuCGUCUAGGA--------ACU-CUUGGuGGUCG- -5' |
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| 7311 | 3' | -50.6 | NC_001900.1 | + | 25438 | 0.73 | 0.495317 |
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Target: 5'- cGAGGCgcaccaucAGcUCCUUGGucgucgcaccggccuGAGCCGCCAGCg -3' miRNA: 3'- -CUUCG--------UCuAGGAACU---------------CUUGGUGGUCG- -5' |
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| 7311 | 3' | -50.6 | NC_001900.1 | + | 39712 | 0.74 | 0.48021 |
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Target: 5'- -cAGCAGAgccagcgCgaUGAGAucaGCCGCCAGCu -3' miRNA: 3'- cuUCGUCUa------GgaACUCU---UGGUGGUCG- -5' |
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| 7311 | 3' | -50.6 | NC_001900.1 | + | 23083 | 0.75 | 0.389107 |
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Target: 5'- cAAGCAGGUcgcCCUUGGGGuugaugccgaggGCCugCAGCa -3' miRNA: 3'- cUUCGUCUA---GGAACUCU------------UGGugGUCG- -5' |
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| 7311 | 3' | -50.6 | NC_001900.1 | + | 26150 | 0.77 | 0.301317 |
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Target: 5'- cGAGCgugcgugcuguagAGAUCCUUGGu-GCCGCCAGCg -3' miRNA: 3'- cUUCG-------------UCUAGGAACUcuUGGUGGUCG- -5' |
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| 7311 | 3' | -50.6 | NC_001900.1 | + | 20726 | 1.13 | 0.001281 |
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Target: 5'- cGAAGCAGAUCCUUGAGAACCACCAGCu -3' miRNA: 3'- -CUUCGUCUAGGAACUCUUGGUGGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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