Results 1 - 20 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7313 | 3' | -63.3 | NC_001900.1 | + | 5808 | 0.66 | 0.347849 |
Target: 5'- -aCCGGCgUCGGCGGUcaUCGAGGUUGg -3' miRNA: 3'- ggGGCCG-GGUCGCUAccGGCUCCAGC- -5' |
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7313 | 3' | -63.3 | NC_001900.1 | + | 43211 | 0.66 | 0.347849 |
Target: 5'- uCCCaCaGCUCcGCGA-GGUgGAGGUCGu -3' miRNA: 3'- -GGG-GcCGGGuCGCUaCCGgCUCCAGC- -5' |
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7313 | 3' | -63.3 | NC_001900.1 | + | 310 | 0.66 | 0.347849 |
Target: 5'- -aCCGGCCCAaCaagGGCCGGGGa-- -3' miRNA: 3'- ggGGCCGGGUcGcuaCCGGCUCCagc -5' |
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7313 | 3' | -63.3 | NC_001900.1 | + | 40381 | 0.66 | 0.347849 |
Target: 5'- aCCaCGGCCgCAGCGGcucacuGCCGAccggccacacGGUCGa -3' miRNA: 3'- gGG-GCCGG-GUCGCUac----CGGCU----------CCAGC- -5' |
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7313 | 3' | -63.3 | NC_001900.1 | + | 27965 | 0.66 | 0.332328 |
Target: 5'- --gCGGCCCAGCGAUcGGaCUGugaaaggagcaAGGUUGg -3' miRNA: 3'- gggGCCGGGUCGCUA-CC-GGC-----------UCCAGC- -5' |
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7313 | 3' | -63.3 | NC_001900.1 | + | 30716 | 0.66 | 0.32476 |
Target: 5'- cCCaCCGGCCC--CGAaaGGCCGgGGGUCc -3' miRNA: 3'- -GG-GGCCGGGucGCUa-CCGGC-UCCAGc -5' |
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7313 | 3' | -63.3 | NC_001900.1 | + | 19996 | 0.66 | 0.32476 |
Target: 5'- cCUUCGGCUCc-CGA-GGCCGuGGUCGc -3' miRNA: 3'- -GGGGCCGGGucGCUaCCGGCuCCAGC- -5' |
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7313 | 3' | -63.3 | NC_001900.1 | + | 17322 | 0.66 | 0.31806 |
Target: 5'- gCCCGaGCCCGGUGAagaacucgccagugcGGUCGAGGa-- -3' miRNA: 3'- gGGGC-CGGGUCGCUa--------------CCGGCUCCagc -5' |
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7313 | 3' | -63.3 | NC_001900.1 | + | 46557 | 0.66 | 0.317322 |
Target: 5'- uCCCCaGCUCguuGGCGAUGGCUu-GGUgGg -3' miRNA: 3'- -GGGGcCGGG---UCGCUACCGGcuCCAgC- -5' |
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7313 | 3' | -63.3 | NC_001900.1 | + | 10503 | 0.66 | 0.310012 |
Target: 5'- gCCUCGGCCU-GCug-GGCaCGAuGGUCGa -3' miRNA: 3'- -GGGGCCGGGuCGcuaCCG-GCU-CCAGC- -5' |
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7313 | 3' | -63.3 | NC_001900.1 | + | 6429 | 0.66 | 0.302831 |
Target: 5'- gCUCgCGGCCaAGCaGAacgcugaGGCUGAGGUCGa -3' miRNA: 3'- -GGG-GCCGGgUCG-CUa------CCGGCUCCAGC- -5' |
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7313 | 3' | -63.3 | NC_001900.1 | + | 25469 | 0.67 | 0.295779 |
Target: 5'- -aCCGGCcugagccgCCAGCG-UGGCUcccacggcccgGAGGUCGu -3' miRNA: 3'- ggGGCCG--------GGUCGCuACCGG-----------CUCCAGC- -5' |
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7313 | 3' | -63.3 | NC_001900.1 | + | 4984 | 0.67 | 0.293688 |
Target: 5'- aCCCCGGCaugcucgucgggcuCCAGCacGgcGGCgGGGGcCGg -3' miRNA: 3'- -GGGGCCG--------------GGUCG--CuaCCGgCUCCaGC- -5' |
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7313 | 3' | -63.3 | NC_001900.1 | + | 23296 | 0.67 | 0.288855 |
Target: 5'- gCCUGcaGCUCGGCGAUGGCagaCGAGuUCGa -3' miRNA: 3'- gGGGC--CGGGUCGCUACCG---GCUCcAGC- -5' |
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7313 | 3' | -63.3 | NC_001900.1 | + | 10575 | 0.67 | 0.288855 |
Target: 5'- aCCaUCGuGCCCAGCa--GGCCGAGGcccaUCGc -3' miRNA: 3'- -GG-GGC-CGGGUCGcuaCCGGCUCC----AGC- -5' |
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7313 | 3' | -63.3 | NC_001900.1 | + | 10242 | 0.67 | 0.275391 |
Target: 5'- gCCCUgauGGCCuCAGCGGaGGCCGGauGUCa -3' miRNA: 3'- -GGGG---CCGG-GUCGCUaCCGGCUc-CAGc -5' |
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7313 | 3' | -63.3 | NC_001900.1 | + | 25229 | 0.67 | 0.268202 |
Target: 5'- aCCCGGCCCGagccucuGCGAggcUGGCUGcGGGa-- -3' miRNA: 3'- gGGGCCGGGU-------CGCU---ACCGGC-UCCagc -5' |
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7313 | 3' | -63.3 | NC_001900.1 | + | 4070 | 0.67 | 0.262433 |
Target: 5'- gCCUGGCUCgaccaccgagacGGUGAUGGCCuucGAGGUg- -3' miRNA: 3'- gGGGCCGGG------------UCGCUACCGG---CUCCAgc -5' |
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7313 | 3' | -63.3 | NC_001900.1 | + | 8169 | 0.67 | 0.262433 |
Target: 5'- uCCCCGGCaCCgAGaCGGUcGCCGAGaaggcguacGUCGa -3' miRNA: 3'- -GGGGCCG-GG-UC-GCUAcCGGCUC---------CAGC- -5' |
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7313 | 3' | -63.3 | NC_001900.1 | + | 6724 | 0.67 | 0.262433 |
Target: 5'- aCCCCgcugcGGCCUuccCGAugUGGCCGucGGUCGa -3' miRNA: 3'- -GGGG-----CCGGGuc-GCU--ACCGGCu-CCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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