Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7320 | 3' | -57.8 | NC_001900.1 | + | 8625 | 0.7 | 0.353772 |
Target: 5'- aCUGGaCGGCUcugGUCGGCuGCcgGGUcUCCg -3' miRNA: 3'- -GGCCaGCCGA---UAGCCG-CGa-CCGaAGG- -5' |
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7320 | 3' | -57.8 | NC_001900.1 | + | 38783 | 0.7 | 0.337493 |
Target: 5'- cUCGG-CGGCUGggcuUCcGUGCaGGCUUCCu -3' miRNA: 3'- -GGCCaGCCGAU----AGcCGCGaCCGAAGG- -5' |
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7320 | 3' | -57.8 | NC_001900.1 | + | 24700 | 0.73 | 0.215586 |
Target: 5'- gCGGUCacacagaucacuGGCgucAUCGGCGCUGGUgguaCCg -3' miRNA: 3'- gGCCAG------------CCGa--UAGCCGCGACCGaa--GG- -5' |
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7320 | 3' | -57.8 | NC_001900.1 | + | 24121 | 0.74 | 0.199375 |
Target: 5'- gCCGGgauagccgccUCGGCaccgagGUUGGCGCgGGCUUCg -3' miRNA: 3'- -GGCC----------AGCCGa-----UAGCCGCGaCCGAAGg -5' |
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7320 | 3' | -57.8 | NC_001900.1 | + | 21619 | 0.74 | 0.189166 |
Target: 5'- aUCGcGUCGGCacAUCGGUGCUcGGCUaCCc -3' miRNA: 3'- -GGC-CAGCCGa-UAGCCGCGA-CCGAaGG- -5' |
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7320 | 3' | -57.8 | NC_001900.1 | + | 23818 | 1.14 | 0.000269 |
Target: 5'- uCCGGUCGGCUAUCGGCGCUGGCUUCCg -3' miRNA: 3'- -GGCCAGCCGAUAGCCGCGACCGAAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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