Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7325 | 5' | -56.2 | NC_001900.1 | + | 25902 | 1.12 | 0.000517 |
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Target: 5'- aCUACGUCCAGCUCCUCCGGUGUGAGGg -3' miRNA: 3'- -GAUGCAGGUCGAGGAGGCCACACUCC- -5' |
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| 7325 | 5' | -56.2 | NC_001900.1 | + | 41591 | 0.79 | 0.115546 |
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Target: 5'- -cACGUCCAGCUUcugggCUCCGGUGUcGGGc -3' miRNA: 3'- gaUGCAGGUCGAG-----GAGGCCACAcUCC- -5' |
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| 7325 | 5' | -56.2 | NC_001900.1 | + | 19873 | 0.75 | 0.221667 |
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Target: 5'- ---aGUCgGGCUCC-CCGGUGUGGGc -3' miRNA: 3'- gaugCAGgUCGAGGaGGCCACACUCc -5' |
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| 7325 | 5' | -56.2 | NC_001900.1 | + | 35795 | 0.72 | 0.343186 |
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Target: 5'- cCUGCGaCCAGUUCUccaggagcaucggcuUCUGGUGUGGGa -3' miRNA: 3'- -GAUGCaGGUCGAGG---------------AGGCCACACUCc -5' |
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| 7325 | 5' | -56.2 | NC_001900.1 | + | 16967 | 0.67 | 0.571641 |
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Target: 5'- -aGCGUUCGGCgCUggagCCGGUGUGGu- -3' miRNA: 3'- gaUGCAGGUCGaGGa---GGCCACACUcc -5' |
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| 7325 | 5' | -56.2 | NC_001900.1 | + | 3685 | 0.67 | 0.593319 |
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Target: 5'- aUGCGUucgaCCAGCUCCUCgGGgucgacGUGGa- -3' miRNA: 3'- gAUGCA----GGUCGAGGAGgCCa-----CACUcc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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