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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7329 | 5' | -58.7 | NC_001900.1 | + | 28728 | 1.05 | 0.000769 |
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Target: 5'- aGACGGCCCGGUCUGUCACCACGACAUc -3' miRNA: 3'- -CUGCCGGGCCAGACAGUGGUGCUGUA- -5' |
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| 7329 | 5' | -58.7 | NC_001900.1 | + | 2259 | 0.68 | 0.363039 |
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Target: 5'- uGACGGCCaGGUCgagGUCA--GCGACGa -3' miRNA: 3'- -CUGCCGGgCCAGa--CAGUggUGCUGUa -5' |
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| 7329 | 5' | -58.7 | NC_001900.1 | + | 8932 | 0.67 | 0.407324 |
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Target: 5'- cGCGGUCCucgaaggcgaGGUCUGgCACgACGGCAa -3' miRNA: 3'- cUGCCGGG----------CCAGACaGUGgUGCUGUa -5' |
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| 7329 | 5' | -58.7 | NC_001900.1 | + | 24970 | 0.66 | 0.484751 |
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Target: 5'- --aGGUCCGGUCUcgGUaCACCAuguCGGCAg -3' miRNA: 3'- cugCCGGGCCAGA--CA-GUGGU---GCUGUa -5' |
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| 7329 | 5' | -58.7 | NC_001900.1 | + | 28904 | 0.66 | 0.454843 |
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Target: 5'- cAUGGCuCCGGUCUGguucggggGCCACGuCAa -3' miRNA: 3'- cUGCCG-GGCCAGACag------UGGUGCuGUa -5' |
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| 7329 | 5' | -58.7 | NC_001900.1 | + | 30584 | 0.66 | 0.454843 |
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Target: 5'- cACGGCCCGGaUCUGcagugcgcuggCACCGCuggugcccGACGUg -3' miRNA: 3'- cUGCCGGGCC-AGACa----------GUGGUG--------CUGUA- -5' |
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| 7329 | 5' | -58.7 | NC_001900.1 | + | 37327 | 0.7 | 0.284904 |
|
Target: 5'- cGACGGCCCuGUCggcGUCcacgaaggcaACCACGACc- -3' miRNA: 3'- -CUGCCGGGcCAGa--CAG----------UGGUGCUGua -5' |
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| 7329 | 5' | -58.7 | NC_001900.1 | + | 31305 | 0.7 | 0.277866 |
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Target: 5'- uGGCuGCCCGGUUcGUCGCUgACGGCAc -3' miRNA: 3'- -CUGcCGGGCCAGaCAGUGG-UGCUGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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