Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 733 | 5' | -54.5 | AC_000020.1 | + | 10766 | 0.66 | 0.579838 |
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Target: 5'- ----cAGUCCGUUAgaAGCCAaggcUUGCCGCa -3' miRNA: 3'- cuuuaUCGGGCAGU--UCGGU----GGCGGUG- -5' |
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| 733 | 5' | -54.5 | AC_000020.1 | + | 21682 | 0.66 | 0.556809 |
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Target: 5'- aGAAG-AGCCCGUCAgugGGaCUACCGUgGa -3' miRNA: 3'- -CUUUaUCGGGCAGU---UC-GGUGGCGgUg -5' |
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| 733 | 5' | -54.5 | AC_000020.1 | + | 10464 | 0.66 | 0.556809 |
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Target: 5'- -cAAUAGUCUGUaacagguuuaaCAGGuCCACCaGCCACc -3' miRNA: 3'- cuUUAUCGGGCA-----------GUUC-GGUGG-CGGUG- -5' |
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| 733 | 5' | -54.5 | AC_000020.1 | + | 23976 | 0.66 | 0.545391 |
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Target: 5'- ----gGGCCCa--AAGCCAgCCGCCGg -3' miRNA: 3'- cuuuaUCGGGcagUUCGGU-GGCGGUg -5' |
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| 733 | 5' | -54.5 | AC_000020.1 | + | 4975 | 0.66 | 0.545391 |
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Target: 5'- ----gGGCCaaagGUCAuGCCACCuCCACc -3' miRNA: 3'- cuuuaUCGGg---CAGUuCGGUGGcGGUG- -5' |
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| 733 | 5' | -54.5 | AC_000020.1 | + | 12044 | 0.66 | 0.53405 |
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Target: 5'- aAAGUAGUCUG--GAGCCAuaGCCGCa -3' miRNA: 3'- cUUUAUCGGGCagUUCGGUggCGGUG- -5' |
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| 733 | 5' | -54.5 | AC_000020.1 | + | 14176 | 0.67 | 0.51163 |
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Target: 5'- ----gGGCCuCGUgGGGCagcaaagggCGCCGCCGCc -3' miRNA: 3'- cuuuaUCGG-GCAgUUCG---------GUGGCGGUG- -5' |
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| 733 | 5' | -54.5 | AC_000020.1 | + | 30613 | 0.7 | 0.341443 |
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Target: 5'- ----gGGUCCGUugcagauaucCAGGCUACCGCCGu -3' miRNA: 3'- cuuuaUCGGGCA----------GUUCGGUGGCGGUg -5' |
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| 733 | 5' | -54.5 | AC_000020.1 | + | 21561 | 1.09 | 0.000482 |
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Target: 5'- cGAAAUAGCCCGUCAAGCCACCGCCACc -3' miRNA: 3'- -CUUUAUCGGGCAGUUCGGUGGCGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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